Decreased viability and neurite length in neural cells treated with chitosan-dextran sulfate nanocomplexes

Autor(es)

Zamproni, Laura N. ^[1] ; Teixeira, Daniela ^[2] ; Alliegro, Amanda A. ^[1] ; Maugeri, Ieda L. ^[2] ; des Rieux, Anne ^[3] ; Porcionatto, Marimelia A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Neurobiol Lab, Rua Pedro de Toledo 669, 3 Andar, BR-04039032 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, Div Immunol, Rua Botucatu 862, BR-04023900 Sao Paulo - Brazil

^[3] Catholic Univ Louvain, Louvain Drug Res Inst, Adv Drug Delivery & Biomat, Ave E Mounier 73, B-1200 Brussels - Belgium

• Tipo de documento: Artigo Científico
• Fonte: NeuroToxicology; v. 76, p. 33-43, JAN 2020.
• Citações Web of Science: 0

Resumo

y CXCL12 is a chemokine known to regulate migration, proliferation, and differentiation of neural stem cells (NSCs) and to play a neuroprotective role in ischemic stroke. Chitosan-dextran sulfate nanocomplexes (Ch/DS NC) are known nanoparticulated systems used to efficiently deliver heparin-binding factors. Here we evaluate Ch/DS NC as carriers for CXCL12 in a mouse model of stroke. Free CXCL12 reduced the size of the ischemic brain lesion. However, when Ch/DS NC were administrated, the stroke volume increased. Neurotoxic screening revealed that Ch/DS NC reduced neuronal viability, decreased the extension of neurites and impaired NSC migration in vitro. To the best of our knowledge, neurotoxicity of Ch/DS NC has not been reported and further screenings will be needed in order to evaluate the biological safety of these nanocomposites. Our results add new data on nanoparticle neurotoxicity and may help us to better understand the complex interactions of the nanostructures with biological components. (AU)

• Processo FAPESP: 12/00652-5 - Estudo dos mecanismos moleculares da migração, sobrevivência e diferenciação de células-tronco neurais
• Beneficiário: Marimélia Aparecida Porcionatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/23797-4 - Hidrogéis contendo nanopartículas com VEGF e CXCL12 para transplante de células-tronco mesenquimais em modelo de traumatismo cranioencefálico
• Beneficiário: Laura Nicoleti Zamproni
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado