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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Decreased viability and neurite length in neural cells treated with chitosan-dextran sulfate nanocomplexes

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Author(s):
Zamproni, Laura N. [1] ; Teixeira, Daniela [2] ; Alliegro, Amanda A. [1] ; Maugeri, Ieda L. [2] ; des Rieux, Anne [3] ; Porcionatto, Marimelia A. [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Neurobiol Lab, Rua Pedro de Toledo 669, 3 Andar, BR-04039032 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, Div Immunol, Rua Botucatu 862, BR-04023900 Sao Paulo - Brazil
[3] Catholic Univ Louvain, Louvain Drug Res Inst, Adv Drug Delivery & Biomat, Ave E Mounier 73, B-1200 Brussels - Belgium
Total Affiliations: 3
Document type: Journal article
Source: NeuroToxicology; v. 76, p. 33-43, JAN 2020.
Web of Science Citations: 0
Abstract

y CXCL12 is a chemokine known to regulate migration, proliferation, and differentiation of neural stem cells (NSCs) and to play a neuroprotective role in ischemic stroke. Chitosan-dextran sulfate nanocomplexes (Ch/DS NC) are known nanoparticulated systems used to efficiently deliver heparin-binding factors. Here we evaluate Ch/DS NC as carriers for CXCL12 in a mouse model of stroke. Free CXCL12 reduced the size of the ischemic brain lesion. However, when Ch/DS NC were administrated, the stroke volume increased. Neurotoxic screening revealed that Ch/DS NC reduced neuronal viability, decreased the extension of neurites and impaired NSC migration in vitro. To the best of our knowledge, neurotoxicity of Ch/DS NC has not been reported and further screenings will be needed in order to evaluate the biological safety of these nanocomposites. Our results add new data on nanoparticle neurotoxicity and may help us to better understand the complex interactions of the nanostructures with biological components. (AU)

FAPESP's process: 12/00652-5 - Molecular mechanisms of neural stem cells migration, survival and differentiation
Grantee:Marimélia Aparecida Porcionatto
Support Opportunities: Regular Research Grants
FAPESP's process: 14/23797-4 - VEGF- and CXCL12-LOADED injectable hydrogel for mesenchymal stem cell delivery in a traumatic brain injury model
Grantee:Laura Nicoleti Zamproni
Support Opportunities: Scholarships abroad - Research Internship - Doctorate