PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis

Autor(es)

Rebech, Gabriela Torres ^[1] ; Venturin, Gabriela Lovizutto ^[1] ; Siqueira Ito, Lucas Takeshi ^[1] ; Bragato, Jaqueline Poleto ^[1] ; de Carvalho Fonseca, Bianca Stefanini ^[1] ; Melo, Larrissa Martins ^[1] ; Costa, Sidnei Ferro ^[1] ; Eugenio, Flavia de Rezende ^[2] ; Patto dos Santos, Paulo Sergio ^[2] ; Felix de Lima, Valeria Marcal ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Sch Vet Med, BR-16050680 Aracatuba - Brazil

^[2] Sao Paulo State Univ, Sch Vet Med, UNESP, Dept Surg & Anim Reprod, Rua Clovis Pestana 793, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 219, JAN 2020.
• Citações Web of Science: 0

Resumo

Leishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future. (AU)

• Processo FAPESP: 13/06684-9 - PD1, apoptose e atividade arginase na leishmaniose visceral canina
• Beneficiário: Valéria Marçal Felix de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular