New tetrodotoxin analogs in Brazilian pufferfishes tissues and microbiome

Autor(es):Mostrar menos -

Chimetto Tonon, Luciane A. ^[1] ; de Azevedo, Gustavo P. R. ^[2] ; Monteiro, Afif F. ^[1] ; Bernardi, I, Darlon ; Gubiani, Juliana R. ^[3] ; Ioca, Laura P. ^[3] ; Mattsson, Hannah K. ^[3] ; Moreira, Ana Paula B. ^[2] ; Gomes, Alexandre F. ^[4] ; Pires Junior, Osmindo R. ^[5] ; Pedrosa, Carolina da S. G. ^[6] ; Souza, Leticia R. Q. ^[6] ; Rehen, Stevens K. ^{[7, 6]} ; Thompson, Cristiane C. ^[2] ; Thompson, Fabiano L. ^[2] ; Berlinck, Roberto G. S. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil

^[2] Fed Univ Rio de Janeiro UFRJ, Inst Biol, Lab Microbiol, SAGE COPPE, Rio De Janeiro, RJ - Brazil

^[3] Bernardi, Darlon, I, Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil

^[4] Waters Technol Brazil, Barueri, SP - Brazil

^[5] Brasilia Univ, Biol Inst, Lab Toxicol, Brasilia, DF - Brazil

^[6] DOr Inst Res & Educ IDOR, Rio De Janeiro, RJ - Brazil

^[7] Fed Univ Rio de Janeiro UFRJ, Inst Biomed Sci, Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 242, MAR 2020.
• Citações Web of Science: 0

Resumo

While tetrodotoxin (TTX) is commonly found in pufferfish tissues, it is unclear if bacterial symbionts isolated from pufferfish tissues can produce TTX. In this investigation, UPLC qTOE-MS/MS analysis of tissue extracts obtained from Sphoeroides spengleri and Canthigaster figuereidoi identified TTX in their composition, indicating their consumption is unsafe. UPLC qTOE-MS/MS analysis coupled with Molecular Networking indicated new TTX analogs (methyl-TTX, TTX-acetate, hydroxypropyl-TTX and glycerol-TTX). Bacterial extracts from sixteen strains revealed a compound with a {[}M+H](+) ion at m/z 320.1088, identical to TTX. However, TTX itself was not detected in these cultures by UPLC-MS/MS. Neurotoxicity of Vibrio A665 purified fraction 2 (with precursor {[}M+H](+) ion at m/z 320.1088) was significant in human neural stem cells (hNSCs), but the Na-v blockage activity was not confirmed by the veratridine/ouabain essays, indicating a possible difference in the mechanism of action between the bacterium A665 purified fraction 2 and TTX. Vibrios symbionts of pufferfish point out involving in the production of TTX precursors. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/50228-8 - Componentes da biodiversidade, e seus caracteres metabólicos, de ilhas do Brasil: uma abordagem integrada
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 16/14375-4 - Ferramentas ômicas aplicadas para descoberta de organismos produtores de tetrodotoxina e genes biossintéticos
• Beneficiário: Luciane Alessandra Chimetto Tonon
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado