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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Association of MFSD3 promoter methylation level and weight regain after gastric bypass: Assessment for 3 y after surgery

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Autor(es):
Nicoletti, Carolina F. [1] ; Pinhel, Marcela S. [1] ; Noronha, Natalia Y. [1] ; Jacome, Amalia [2] ; Crujeiras, Ana B. [3, 4, 5] ; Barbosa Nonino, Carla [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Lab Nutrigen Studies, Sao Paulo, SP - Brazil
[2] Univ A Coruna, Fac Sci, CITIC, Dept Math, MODES Grp, La Coruna - Spain
[3] Univ Clin Hosp Santiago CHUS SERGAS, Hlth Res Inst Santiago IDIS, Epigen Endocrinol & Nutr, Santiago De Compostela - Spain
[4] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Madrid - Spain
[5] Santiago Compostela Univ USC, Santiago De Compostela - Spain
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: NUTRITION; v. 70, FEB 2020.
Citações Web of Science: 0
Resumo

Objectives: Genetic predisposition and epigenetic signatures could explain why some individuals regain the weight lost after different obesity treatments. Major facilitator superfamily domain 3 (MFSD3) is a family of membrane-bound solute carriers whose expression has been recently associated with nutrient intake and adipose tissue homeostasis. This study aimed to evaluate a possible association between MFSD3 preoperative methylation pattern and weight regain after bariatric surgery. Methods: This is a longitudinal study comprising 24 obese (body mass index > 35 m/kg(2)) women submitted to gastric bypass. Anthropometric measurements were evaluated at preoperative time and 1, 2, and 3 y after surgery, and then weight regain was calculated. Genomic DNA was extracted from leukocytes and was bisulfite modified by specific kits, according manufacturer's instructions. Methylation analysis was performed with the Infinium Human Methylation 450 K bead chip technology, and methylation level was expressed as a beta value ranging from 0 (unmethylated) to 1 (fully methylated). Shapiro-Wilk, repeated-means analysis of variance, Spearman correlation, Mann-Whitney, and independent t tests were used in statistical analysis (P < 0.05). Results: A total of 25% of patients regained significant weight. Weight regain after bariatric surgery was positively correlated with cg00010266 MFSD3 preoperative methylation levels (r = 0.6804, P = 0.0126). Moreover, cg00010266 MFSD3 baseline methylation levels were significantly higher in regainer patients than non-regainers (6.2 +/- 1.5 versus 3.9 +/- 1.2%, P = 0.026). Patients allocated in the higher cg00010266 MFSD3 preoperative methylation group had higher weight regain (4.1 +/- 1.8 versus 6.7 +/- 2.2 kg, P = 0.037). Conclusions: Preoperative hypermethylation of MFSD3 gene is significantly associated with weight regain and a worse response to gastric bypass. (C) 2019 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 17/07220-7 - Aplicação de ferramentas de bioinformática para análise do perfil de metilação do DNA antes e após a cirurgia bariátrica
Beneficiário:Carolina Nicoletti Ferreira Fino
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 16/05638-1 - Padrão de metilação global do DNA antes e após tratamentos para perda de peso
Beneficiário:Carla Barbosa Nonino
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/18669-0 - Avaliação do padrão de metilação do DNA antes e após derivação gástrica em Y-de-Roux
Beneficiário:Carolina Nicoletti Ferreira Fino
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado