IncX4 Plasmid-Mediated mcr-1.1 in Polymyxin-Resistant Escherichia coli from Outpatients in Santa Catarina, Southern Brazil

Autor(es):Mostrar menos -

Zamparette, Caetana P. ^[1] ; Schorner, Marcos ^[1] ; Campos, Elizandra ^[1] ; Moura, Quezia ^[2] ; Cerdeira, Louise ^[2] ; Tartari, Daniela C. ^[1] ; Sereia, Aline F. R. ^[3] ; Cunha, Patricia ^[3] ; Fontana, Herrison ^[2] ; de Oliveira, Luiz Felipe V. ^[3] ; Grisard, Edmundo C. ^[4] ; Lincopan, Nilton ^[2] ; Bazzo, Maria Luiza ^[1] ; Sincero, Thais C. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Catarina, Hlth Sci Ctr, Dept Clin Anal, BR-88040900 Florianopolis, SC - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[3] Neoprospecta Microbiome Technol, Florianopolis, SC - Brazil

^[4] Univ Fed Santa Catarina, Ctr Biol Sci, Dept Microbiol Imunol & Parasitol, Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIAL DRUG RESISTANCE; v. 26, n. 11 JAN 2020.
• Citações Web of Science: 1

Resumo

Plasmid-mediated polymyxin resistance has become a global health concern, not only because its dissemination has occurred drastically but also because it has begun to be reported in multidrug-resistant (MDR) pathogens. We hereby report microbiological and genomic characteristics of two mcr-1.1-positive polymyxin-resistant Escherichia coli isolates identified for the first time in community patients, in Santa Catarina, Southern Brazil. E. coli strains belonging to ST206 and ST354 and the resistome analysis revealed the presence of clinically important genes responsible for MDR profile. Interestingly, in both polymyxin-resistant E. coli strains, mcr-1.1 genes were carried by IncX4 plasmids, responsible for the worldwide dissemination of mcr-type genes. In this regard, plasmid backbones were almost identical to the first IncX4 plasmid reported in Brazil and sharing more than 99.9% identity to IncX4 plasmids from China, also lacking the ISApl1 insertion sequence upstream of mcr-1. In conclusion, these data confirm the presence of international ST206 and ST354 carrying mcr-1.1 genes and that the IncX4 plasmids have been key vectors contributing to the endemic status of mcr-1.1-positive polymyxin-resistant E. coli in Brazil. Also, we described the first known clinical isolate with the mrc1.1 gene in Santa Catarina state, Brazil, showing that plasmid-mediated polymyxin resistance has been affecting humans earlier than has been known so far. (AU)

• Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular