Proneural and mesenchymal glioma stem cells display major differences in splicing and lncRNA profiles

Autor(es)

Guardia, Gabriela D. A. ^[1] ; Correa, Bruna R. ^{[1, 2]} ; Araujo, Patricia Rosa ^[3] ; Qiao, Mei ^[3] ; Burns, Suzanne ^[3] ; Penalva, Luiz O. F. ^{[3, 4]} ; Galante, Pedro A. F. ^[1]

Afiliação do(s) autor(es)

^[1] Hosp Sirio Libanes, Ctr Oncol Mol, BR-01309060 Sao Paulo, SP - Brazil

^[2] Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Barcelona 08003, Catalonia - Spain

^[3] UT Hlth San Antonio, Childrens Canc Res Inst, San Antonio, TX 78229 - USA

^[4] UT Hlth San Antonio, Dept Cell Syst & Anat, San Antonio, TX 78229 - USA

• Tipo de documento: Artigo Científico
• Fonte: NPJ GENOMIC MEDICINE; v. 5, n. 1 JAN 16 2020.
• Citações Web of Science: 0

Resumo

Therapy resistance and recurrence in high-grade gliomas are driven by their populations of glioma stem cells (GSCs). Thus, detailed molecular characterization of GSCs is needed to develop more effective therapies. We conducted a study to identify differences in the splicing profile and expression of long non-coding RNAs in proneural and mesenchymal GSC cell lines. Genes related to cell cycle, DNA repair, cilium assembly, and splicing showed the most differences between GSC subgroups. We also identified genes distinctly associated with survival among patients of mesenchymal or proneural subgroups. We determined that multiple long non-coding RNAs with increased expression in mesenchymal GSCs are associated with poor survival of glioblastoma patients. In summary, our study established critical differences between proneural and mesenchymal GSCs in splicing profiles and expression of long non-coding RNA. These splicing isoforms and lncRNA signatures may contribute to the uniqueness of GSC subgroups, thus contributing to cancer phenotypes and explaining differences in therapeutic responses. (AU)

• Processo FAPESP: 18/15579-8 - Retroelementos: uma força motriz criando novidades genéticas no genoma humano e de camundongos
• Beneficiário: Pedro Alexandre Favoretto Galante
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 13/07159-5 - Proteínas de ligação a RNA e variações pós-transcricionais: influência no desenvolvimento de glioblastomas multiformes
• Beneficiário: Bruna Renata Silva Corrêa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/19541-2 - Impacto de proteínas de ligação a RNA na desregulação de splicing em glioblastoma
• Beneficiário: Gabriela Der Agopian Guardia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/25483-4 - Identificação e análise funcional de proteínas de ligação a RNA associadas com o desenvolvimento do glioblastoma multiforme
• Beneficiário: Bruna Renata Silva Corrêa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado