Effect of cell therapy with osteoblasts differentiated from bone marrow or adipose tissue stromal cells on bone repair

Autor(es)

Freitas, Gileade P. ^[1] ; Lopes, Helena B. ^[1] ; Souza, Alann T. P. ^[1] ; Oliveira, Paula G. F. P. ^[1] ; Almeida, Adriana L. G. ^[1] ; Coelho, Paulo G. ^{[2, 3]} ; Ferreira, Fernanda U. ^[4] ; Covas, Dimas T. ^{[5, 4]} ; Beloti, Marcio M. ^[6] ; Rosa, Adalberto L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Sao Paulo, SP - Brazil

^[2] NYU, Sch Med, Hansjorg Wyss Dept Plast Surg, New York, NY 10016 - USA

^[3] NYU, Coll Dent, Dept Biomat, New York, NY 10010 - USA

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Res, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Clin Med, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REGENERATIVE MEDICINE; v. 14, n. 12, p. 1107-1119, DEC 2019.
• Citações Web of Science: 0

Resumo

Aim: The aim of this study was to investigate the effect of local injection of osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on bone tissue formation. Materials \& methods: Defects were created in rat calvaria and injected with BM-OB or AT-OB and phosphate-buffered saline without cells were injected as control. Bone formation was evaluated 4 weeks postinjection. Results: Injection of BM-OB or AT-OB resulted in higher bone formation than that obtained with control. The bone tissue induced by cell injections exhibited similar mechanical properties as those of pristine calvarial bone, and its molecular cues suggested the occurrence of a remodeling process. Conclusion: Results of this study demonstrated that cell therapy with osteoblasts induced significant bone formation that exhibited the same quality as that of pre-existent bone. (AU)

• Processo FAPESP: 14/26864-4 - Efeito de Injeções Locais de Células-Tronco Mesenquimais ou Osteoblastos na Regeneração do Tecido Ósseo
• Beneficiário: Gileade Pereira Freitas
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/21439-6 - Células-tronco mesenquimais e osteoblastos: avaliação das assinaturas moleculares e do potencial para regenerar tecido ósseo
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54142-5 - EMU: criação do Laboratório Multiusuários para análises de imagens tridimensionais de tecidos biológicos e biomateriais (LAB 3D BIO)
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários