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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Electrical field stimulation induces endothelium-dependent contraction of human umbilical cord vessels

Texto completo
Autor(es):
Britto-Junior, Jose [1] ; Jacintho, Felipe Fernandes [1] ; Figueiredo Murari, Guilherme M. [1] ; Campos, Rafael [2] ; Moreno, Ronilson Agnaldo [1] ; Antunes, Edson [1] ; Monica, Fabiola Z. [1] ; De Nucci, Gilberto [3, 1, 4]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] State Univ Campinas Unicamp, Fac Med Sci, Dept Pharmacol, Campinas - Brazil
[2] Ceara State Univ UECE, Super Inst Biomed Sci, Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
[4] Metropolitan Univ Santos UNIMES, Santos, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Life Sciences; v. 243, FEB 15 2020.
Citações Web of Science: 0
Resumo

Electrical field stimulation (EFS) has been used for decades in classical pharmacological preparations in order to characterize the mediators released by neural endings involved in smooth muscle contraction or relaxation. Since most of the human umbilical cord has no innervation, EFS has never been used in this preparation. This study aimed to investigate the effect of EFS on vascular responsiveness from human umbilical cord. Segments of the human umbilical cord were obtained from normotensive parturients and the human umbilical artery (HUA) and the human umbilical vein (HUV) were isolated and mounted in organ bath chambers. Electrical field stimulation-induced contractions in both HUA (2.35 +/- 1.31 mN and 3.77 +/- 2.31 mN for 8 Hz and 16 Hz respectively, n = 24) and HUV (3.81 +/- 2.54 mN and 6.26 +/- 4.51 mN for 8 Hz and 16 Hz respectively, n = 25). The addition of tetrodotoxin (1 mu M) did not alter the EFS-induced contractions in both tissues (n = 5). Pre-incubation with atropine (10 and 100 mu M), glibenclamide (10 mu M) and indomethacin (10 mu M) did not affect the EFS-induced contractions in both tissues. The contractions of both vessels were significantly reduced by pre-incubation of the tissues with phentolamine (10 and 100 mu M). The endothelium removal almost abolished the EFS-induced contractions in both vessels (n = 5). In sandwich preparation, donor tissue (with endothelium) released a factor (s) that promoted contraction of the recipient tissue (endothelium removal) in both HUA and HUV (n = 5, respectively). Our findings indicate a potential role of endothelium-derived catecholamines in modulating HUA and HUV reactivities. (AU)

Processo FAPESP: 18/24971-9 - Relevância fisiológica das catecolaminas produzidas pelo endotélio
Beneficiário:Felipe Fernandes Jacintho
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata
Beneficiário:Edson Antunes
Modalidade de apoio: Auxílio à Pesquisa - Temático