Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva

Autor(es)

Araujo, Heitor Ceolin ^{[1, 2]} ; Nakamune, Ana Claudia Melo Stevanato ^{[3, 4]} ; Garcia, Wilson Galhego ^[3] ; Pessan, Juliano Pelim ^{[1, 2]} ; Antoniali, Cristina ^{[3, 1, 2, 4]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Grad Program Dent Sci, Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Pediat Dent & Publ Hlth, Sao Paulo - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Dent, Dept Basic Sci, Sao Paulo - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Multictr Grad Program Physiol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2020, JAN 29 2020.
• Citações Web of Science: 0

Resumo

Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS (TM) criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage. (AU)

• Processo FAPESP: 16/22180-9 - Avaliação de mecanismos de modificação pós-translacionais que aumentam a biodisponibilidade do óxido nítrico em vasos de ratas normotensas e espontaneamente hipertensas SHR)ao final da prenhez
• Beneficiário: Cristina Antoniali Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular