Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms

Autor(es)

Fidelis-de-Oliveira, Patricia ^[1] ; Aparecida-Castro, Samanta ^[1] ; Silva, Denise Brentan ^[2] ; de Melo Morais, Ingrid Beatriz ^[3] ; Martins de Miranda, Victor Hugo ^[1] ; de Gobbi, Juliana Irani ^[1] ; Nunes Canabrava, Hudson Armando ^[3] ; Bispo-da-Silva, Luiz Borges ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Physiol, Inst Biosci, Botucatu, SP - Brazil

^[2] Fed Univ Mato Grosso Sul UFMS, Lab Prod Nat & Espectrometria Massas LAPNEM, Campo Grande, MS - Brazil

^[3] Fed Univ Uberlandia UFU, Dept Pharmacol, Inst Biomed Sci, Campus Umuarama, Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 251, APR 6 2020.
• Citações Web of Science: 1

Resumo

Ethnopharmacological relevance: Eugenia dysenterica (ED) leaves are used in Brazil to treat cardiac diseases; however, there are no scientific data describing the effects of this species on cardiac activity. Aim of the study: To investigate the effect of ED aqueous leaf extract (EDLE) on hear rate (HR) and mean arterial pressure (MAP) of anaesthetised rats and its underlying mechanism of action. Material and methods: EDLE was analysed, and its proanthocyanidin composition was determined. After performing dose-effect curves for EDLE on HR and MAP, EDLE-induced hypotension was evaluated before and after atropine (AT), L-N(omega)-nitro-L-arginine methyl ester (L-NAME), hexamethonium (HXT), indomethacin (IND), carbenoxolone (CBX), or nifedipine (NFD) administration. The effect of proanthocyanidin-depleted extract (EDLE/P-) was also determined and compared to that of the EDLE with proanthocyanidins. Results: EDLE decreased the MAP in a dose-dependent manner; HR was decreased only with the highest and most toxic dose. Only CBX and NFD decreased EDLE-induced hypotension. Five polymeric series of proanthocyanidins were identified, which were mainly constituted by procyanidin and prodelphinidin units with B-type linkage and up to 12 flavan-3-ol units. EDLE/P- induced hypotension did not differ from that induced by EDLE. Conclusions: The cardiovascular effects of EDLE were primarily related to its vascular action. EDLE-induced hypotensive effect appeared to involve L-type calcium channel blockage as well as myoendothelial gap junction signalling. The higher molecular weight proanthocyanidins from EDLE are unlikely to contribute to its cardiovascular effect. (AU)

• Processo FAPESP: 13/18382-7 - Avaliação dos efeitos de extrato aquoso liofilizado das folhas de Eugenia dysenterica DC sobre o sistema cardiovascular
• Beneficiário: Samanta Aparecida Castro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica