Hyperglycemic condition influence on mineral trioxide aggregate biocompatibility and biomineralization

Autor(es)

de Azevedo Queiroz, India O. ^[1] ; Goncalves, Luanna O. ^[1] ; Takamiya, Aline S. ^[2] ; Rezende, Gabriely C. ^[1] ; Machado, Thiago ^{[3, 4]} ; Siveiri-Araujo, Gustavo ^[1] ; Ervolino, Edilson ^[5] ; Oliveira, Sandra H. P. ^[5] ; Gomes-Filho, Joao E. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Aracatuba Dent Sch, Dept Endodont, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba - Brazil

^[3] UNESP, Aracatuba Dent Sch, Dept Oral & Maxillofacial Surg, Aracatuba - Brazil

^[4] UNESP, Aracatuba Dent Sch, Integrated Clin, Aracatuba - Brazil

^[5] UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 108, n. 3, p. 663-673, APR 2020.
• Citações Web of Science: 0

Resumo

This study aimed to investigate in vitro and in vivo the influence of hyperglycemic condition on biocompatibility and biomineralization of gray mineral trioxide aggregate (GMTA) and white mineral trioxide aggregate (WMTA). For the in vitro study, fibroblast-like cells L929 were cultured under high or normal glucose concentration to investigate the effects of both MTA's on cell proliferation and inflammatory cytokines production IL-1 beta, IL-6, and TNF-alpha. For the in vivo study, polyethylene tubes containing MTA materials and empty tubes were implanted into dorsal connective tissues of Wistar rats previously assigned normal and hyperglycemic. After 7 and 30 days, the tubes with surrounding tissues were removed and subjected to histological, fluorescence and immunohistochemical analyzes of IL-1 beta, IL-6, and TNF-alpha. In vitro study showed that, under high glucose condition, GMTA reduced cell proliferation and IL-6 production compared with WMTA. Moreover, in vivo study revealed that hyperglycemic condition did not modify the inflammatory response and cytokines production in the tissue close to both materials. Independently of hyperglycemic status, mineralized areas were observed with both materials, but the fluorescence intensity of WMTA was diminished on 14 days in hyperglycemic animals. It is possible to conclude that GMTA was able to inhibit the proliferation rate and IL-6 production under high glucose concentration in vitro. Furthermore, cytokines production and inflammatory response were not upregulated in hyperglycemic animals; however, a decrease in the calcium deposition was observed in presence of WMTA, suggesting a delay in the mineralization process. (AU)

• Processo FAPESP: 14/25475-4 - Expressão de IL1-B e TNF-a na resposta tecidual ao MTA em animais diabéticos
• Beneficiário: Luanna de Oliveira Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/06641-8 - Avaliação comparativa da resposta tecidual de ratos diabéticos ao MTA cinza e MTA branco
• Beneficiário: Índia Olinta de Azevedo Queiroz
• Modalidade de apoio: Bolsas no Brasil - Doutorado