Structural and Functional Stabilization of Sericin from Bombyx mori Cocoons in a Biopolysaccharide Film: Bioorigami for Skin Regeneration

Autor(es)

Balcao, Victor M. ^{[1, 2, 3]} ; Harada, Liliam K. ^[2] ; Jorge, Ludmilla R. ^[2] ; Oliveira Jr, Jose M. ; Tubino, Matthieu ^[4] ; Vila, Marta M. D. C. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Aveiro, Dept Biol, Campus Santiago, P-3810193 Aveiro - Portugal

^[2] Univ Sorocaba, Lab Biofilmes & Bacteriofagos, BR-18023000 Sorocaba, SP - Brazil

^[3] Univ Aveiro, Ctr Environm & Marine Studies, Campus Santiago, P-3810193 Aveiro - Portugal

^[4] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil

^[5] Oliveira Jr, Jr., Jose M., Univ Sorocaba, Lab Biofilmes & Bacteriofagos, BR-18023000 Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 31, n. 4, p. 833-848, APR 2020.
• Citações Web of Science: 0

Resumo

Development and optimization of a bioorigami film with silk sericin was pursued for skin regeneration. Several formulations were produced, with varying integrated sericin contents, viz. 0, 1, 2, 5, 10, 20 and 50 mgsericin mLfilm-1. The physico-chemical characteristics of the bioorigami films produced were evaluated via infrared spectrophotometry, X-ray diffraction, X-ray transmission, X-ray fluorescence, thermogravimetry, differential scanning calorimetry, transdermal protein permeation, kinetics of protein release from the bioorigami films and free radical scavenging activities. The mechanical resistance was also evaluated. The results by 2,2-diphenyl-1-picryl-hydrazil (DPPH) assay suggest that the bioorigami films integrating crude sericin extract had capacity to sequester free radicals, exhibited prolonged release of the bioactive protein, good physico-chemical characteristics and adequate mechanical resistance. Specifically, the bioorigami film with 10 mgsericin mLfilm-1 proved to be able to release all the bioactive protein in a 12 h timeframe. The results suggest potential for use in biopharmaceutical application, specifically, as material for use in hydrogels. (AU)

• Processo FAPESP: 16/16641-3 - Estabilização estrutural e funcional de sericina em hidrogel biopolissacarídico: bio-origami para a regeneração de pele
• Beneficiário: Liliam Katsue Harada Rocha
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/05522-9 - PsaPhageKill - isolamento, caracterização e uso de bacteriófagos líticos contra Pseudomonas syringae pv. actinidiae para combater o cancro do kiwi: uma alternativa eficiente e amiga do ambiente
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 16/12234-4 - TransAppIL - Permeação cutânea de entidades proteicas estabilizadas estruturalmente e funcionalmente, carreadas por líquidos iônicos.
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/08884-3 - PneumoPhageColor - desenvolvimento de um kit de biodetecção colorimétrica de Pseudomonas aeruginosa com base em partículas fágicas
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular