| Full text | |
| Author(s): |
Balcao, Victor M.
[1, 2, 3]
;
Harada, Liliam K.
[2]
;
Jorge, Ludmilla R.
[2]
;
Oliveira Jr, Jose M.
;
Tubino, Matthieu
[4]
;
Vila, Marta M. D. C.
[5]
Total Authors: 6
|
| Affiliation: | [1] Univ Aveiro, Dept Biol, Campus Santiago, P-3810193 Aveiro - Portugal
[2] Univ Sorocaba, Lab Biofilmes & Bacteriofagos, BR-18023000 Sorocaba, SP - Brazil
[3] Univ Aveiro, Ctr Environm & Marine Studies, Campus Santiago, P-3810193 Aveiro - Portugal
[4] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil
[5] Oliveira Jr, Jr., Jose M., Univ Sorocaba, Lab Biofilmes & Bacteriofagos, BR-18023000 Sorocaba, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Journal of the Brazilian Chemical Society; v. 31, n. 4, p. 833-848, APR 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Development and optimization of a bioorigami film with silk sericin was pursued for skin regeneration. Several formulations were produced, with varying integrated sericin contents, viz. 0, 1, 2, 5, 10, 20 and 50 mgsericin mLfilm-1. The physico-chemical characteristics of the bioorigami films produced were evaluated via infrared spectrophotometry, X-ray diffraction, X-ray transmission, X-ray fluorescence, thermogravimetry, differential scanning calorimetry, transdermal protein permeation, kinetics of protein release from the bioorigami films and free radical scavenging activities. The mechanical resistance was also evaluated. The results by 2,2-diphenyl-1-picryl-hydrazil (DPPH) assay suggest that the bioorigami films integrating crude sericin extract had capacity to sequester free radicals, exhibited prolonged release of the bioactive protein, good physico-chemical characteristics and adequate mechanical resistance. Specifically, the bioorigami film with 10 mgsericin mLfilm-1 proved to be able to release all the bioactive protein in a 12 h timeframe. The results suggest potential for use in biopharmaceutical application, specifically, as material for use in hydrogels. (AU) | |
| FAPESP's process: | 16/16641-3 - Structural and functional stabilization of sericin in a biopolysaccharide hydrogel: bio-origami for skin regeneration |
| Grantee: | Liliam Katsue Harada Rocha |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative |
| Grantee: | Vitor Manuel Cardoso Figueiredo Balcão |
| Support Opportunities: | Scholarships abroad - Research |
| FAPESP's process: | 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids. |
| Grantee: | Marta Maria Duarte Carvalho Vila |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles |
| Grantee: | Vitor Manuel Cardoso Figueiredo Balcão |
| Support Opportunities: | Regular Research Grants |