Determination of isotretinoin (13-cis-retinoic acid) using a sensor based on reduced graphene oxide modified with copper nanoparticles

Autor(es)

Fernandes, Victoria Quintas ^[1] ; Leme Silva, Martin K. ^[1] ; Cesarino, Ivana ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Agr, UNESP, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ELECTROANALYTICAL CHEMISTRY; v. 856, JAN 1 2020.
• Citações Web of Science: 0

Resumo

The electrochemical behaviour of isotretinoin (13-cis-RA) on an electrode modified with a composite based on reduced graphene oxide (rGO) modified with copper nanoparticles (CuNPs) was investigated. The rGO-CuNPs composite was successfully synthesized by a simple chemical reduction step and then characterized by microscopic and electrochemical techniques. The rGO-CuNPs modified glassy carbon (GC) electrode was applied successfully in the direct electrochemical detection of isotretinoin in a 0.2 mol L-1 phosphate buffer solution (PBS - pH 7.0) solution (as the electrolyte). Differential pulse voltammetry (DPV) was the methodology used. The presented method displayed linearity in the concentration range from 02 to 3.0 mu mol L-1 with a limit of detection (LOD) of 15.3 nmol L-1. This is the lowest LOD for isotretinoin reported in the literature using an electroanalytical approach. The proposed sensor was applied in the determination of isotretinoin in synthetic plasmatic serum with a recovery of 91% from the original signal. (C) 2019 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/24274-3 - Desenvolvimento de biossensor de glicose como ferramenta analítica visando a otimização da produção de etanol de segunda geração a partir de resíduos do abacaxizeiro
• Beneficiário: Ivana Cesarino
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 17/08733-8 - Avaliação do compósito baseado em grafeno modificado com diferentes nanopartículas metálicas no desenvolvimento de um sensor eletroquímico para determinação de isotretinoína
• Beneficiário: Victória Quintas Fernandes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica