Imbalanced testicular metabolism induced by thyroid disorders: New evidences from quantitative proteome

Autor(es):Mostrar menos -

Gomes, Samantha Nascimento ^[1] ; do Carmo Correa, Deborah Elzita ^[1] ; de Oliveira, Isabela Medeiros ^[1] ; Bargi-Souza, Paula ^[2] ; Cavallin, Monica Degraf ^[1] ; Mariano, Danielle Dobner ^[1] ; Khalil, Najeh Maissar ^[3] ; Alves Figueiredo, David Livingstone ^[1] ; Romano, Marco Aurelio ^[1] ; de Oliveira, Claudio Alvarenga ^[4] ; Romano, Renata Marino ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Ctr Oeste UNICENTRO, Grp Estudo & Pesquisa Tireoide GEPET, Dept Med, Rua Simeao Camargo Varela de Sa 03, BR-85040080 Guarapuava, Parana - Brazil

^[2] Univ Fed Minas Gerais, Dept Physiol & Biophys, Inst Biol Sci, Ave Presidente Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

^[3] State Univ Ctr Oeste UNICENTRO, Pharmaceut Nanotechnol Lab, Dept Pharm, Rua Simeao Camargo Varela da Sa 03, BR-85040080 Guarapuava, Parana - Brazil

^[4] Univ Sao Paulo, Lab Hormonal Dosages, Dept Anim Reprod, Fac Vet Med, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENDOCRINE; v. 67, n. 1, p. 209-223, JAN 2020.
• Citações Web of Science: 0

Resumo

Thyroid dysfunctions, such as hypothyroidism and hyperthyroidism, are the second most prevalent endocrinopathies and are associated to reproductive disorders in men. Several genes are differentially modulated by thyroid hormones in testes and imbalances in thyroid hormone levels are also associated to alterations on sperm functionality. Imbalances on antioxidant defense mechanism and stress oxidative have been pointed out as the main factors for the impairments on male reproductive function. To clarify this issue, we investigated the expression and activity of antioxidant enzymes in testis, followed by their proteomic profile in attempt to characterize the mechanisms involved in the alterations induced by hypo-or hyperthyroidism in adult male rats. Hypothyroidism reduced the Gsr transcript expression and the activity of CAT and GSR enzymes, while the hyperthyroidism reduced the Gpx4 var2 transcript expression. Among 1082 identified proteins, 123 and 37 proteins were downregulated by hypothyroidism compared to euthyroid and hyperthyroid condition, respectively, being 36 proteins commonly reduced in both comparisons and one exclusively in hypo-hyperthyroidism comparison. A network containing 29 nodes and 68 edges was obtained in protein-protein interaction analysis and the functional enrichment analysis of differentially expressed proteins revealed significant alterations for several functions in hypo-euthyroid and hypo-hyperthyroid comparisons, such as ATP metabolic process, coenzyme binding, sperm part, peroxiredoxin activity, mitochondrial protein complex, intramolecular oxidoreductase activity, binding of sperm to zona pellucida, glutathione transferase activity, response to testosterone. Thus, there is a correlation between thyroid disorders and impaired antioxidant defense mechanism, resulting in reproductive dysfunctions, as infertility, mainly observed in hypothyroidism. (AU)

• Processo FAPESP: 14/10845-0 - Consequências do hipotireoidismo e da tirotoxicose desenvolvidos na vida adulta sobre a função gonadal masculina: expressão gênica de marcadores moleculares e perfil proteômico em tecidos do eixo hipotalâmico-hipofisário-gonadal
• Beneficiário: Claudio Alvarenga de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular