Factor XII-Deficient Chicken Plasma as a Useful Target for Screening of Pro- and Anticoagulant Animal Venom Toxins

Autor(es)

Prezoto, Benedito C. ^[1] ; Oguiura, Nancy ^[2]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Pharmacol Lab, Ave Dr Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

^[2] Butantan Inst, Ecol & Evolut Lab, Ave Dr Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 12, n. 2 FEB 2020.
• Citações Web of Science: 0

Resumo

The sensitivity of vertebrate citrated plasma to pro- and anticoagulant venom or toxins occurs on a microscale level (micrograms). Although it improves responses to agonists, recalcification triggers a relatively fast thrombin formation process in mammalian plasma. As it has a natural factor XII deficiency, the recalcification time (RT) of chicken plasma (CP) is comparatively long {[}>= 1800 seconds (s)]. Our objective was to compare the ability of bee venom phospholipase A(2) (bvPLA(2)) to neutralize clot formation induced by an activator of coagulation (the aPTT clot) in recalcified human and chicken plasmas, through rotational thromboelastometry. The strategy used in this study was to find doses of bvPLA(2) that were sufficient enough to prolong the clotting time (CT) of these activated plasmas to values within their normal RT range. The CT of CP was prolonged in a dose-dependent manner by bvPLA(2), with 17 +/- 2.8 ng (n = 6) being sufficient to displace the CT values of the activated samples to >= 1800 s. Only amounts up to 380 +/- 41 ng (n = 6) of bvPLA(2) induced the same effect in activated human plasma samples. In conclusion, the high sensitivity of CP to agonists and rotational thromboelastometry could be useful. For example, during screening procedures for assaying the effects of toxins in several stages of the coagulation pathway, such as clot initiation, formation, stability, strength, or dissolution. (AU)

• Processo FAPESP: 09/54620-4 - Avaliação da atividade pró-coagulante do ácido ribonucléico (RNA) sobre a via intrínseca da coagulação sanguínea em mamíferos e aves
• Beneficiário: Benedito Carlos Prezoto
• Modalidade de apoio: Auxílio à Pesquisa - Regular