Effects of copper oxide nanoparticles on growth of lettuce (Lactuca sativa L.) seedlings and possible implications of nitric oxide in their antioxidative defense

Autor(es)

Pelegrino, Milena Trevisan ^[1] ; Kohatsu, Marcio Yukihiro ^[2] ; Seabra, Amedea Barozzi ^[1] ; Monteiro, Lucilena Rebelo ^[3] ; Gomes, Diego Genuario ^[4] ; Oliveira, Halley Caixeta ^[4] ; Rolim, Wallace Rosado ^[1] ; de Jesus, Tatiane Araujo ^[2] ; Batista, Bruno Lemos ^[1] ; Lange, Camila Neves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

^[2] Univ Fed ABC, Ctr Engn Modelagem & Ciencias Sociais Aplicadas, Santo Andre, SP - Brazil

^[3] Comissao Nacl Energia Nucl, Ctr Quim & Meio Ambiente, Ipen, CNEN, SP, Inst Pesquisas Energet & Nucl, Sao Paulo, SP - Brazil

^[4] Univ Estadual Londrina, Dept Anim & Plant Biol, Londrina, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL MONITORING AND ASSESSMENT; v. 192, n. 4 MAR 12 2020.
• Citações Web of Science: 12

Resumo

Copper oxide nanoparticles (CuO NPs) have been extensively explored for use in agriculture. Previous studies have indicated that application of CuO NPs might be promising for development and conservation of plants, pest control, and for the recovery of degraded soils. However, depending on the applied concentration copper can cause phytotoxic effects. In this work, biosynthesized CuO NPs (using green tea extract) were evaluated on their effects on lettuce (Lactuca sativa L.) seedling growth, which were exposed at concentrations ranged between 0.2 and 300 mu g mL(-1). From the biosynthesized were obtained ultra-small CuO NPs (similar to 6.6 nm), with high stability in aqueous suspension. Toxicity bioassays have shown that at low concentrations (up to 40 mu g mL(-1)), CuO NPs did not affect or even enhanced the seed germination. At higher concentrations (higher than 40 mu g mL(-1)), inhibition of seed germination and radicle growth ranging from 35 to 75% was observed. With the increase of CuO NPs concentrations, nitrite and S-nitrosothiols levels in radicles increased, whereas superoxide dismutase and total antioxidant activities decreased. The nitrite and S-nitrosothiols levels in lettuce radicles showed a direct dose response to CuO NP application, which may indicate nitric oxide-dependent signaling pathways in the plant responses. Therefore, the results demonstrated that at low concentrations (<= 20 mu g mL(-1)) of CuO NPs, beneficial effects are obtained from seedlings, enhancing plant growth, and the involvement of nitric oxide signaling in the phytotoxic effects induced by high concentration of this formulation. (AU)

• Processo FAPESP: 18/08194-2 - Óleo essencial contendo nanopartículas metálicas funcionalizadas com óxido nítrico como estratégia para o controle de patógenos vegetais na agricultura
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/05029-8 - Preparação, caracterização e ensaios biológicos de biomateriais poliméricos doadores de óxido nítrico
• Beneficiário: Milena Trevisan Pelegrino
• Modalidade de apoio: Bolsas no Brasil - Doutorado