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Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature

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Autor(es):
Lídia J. Tasima ; Caroline Serino-Silva ; Daniela M. Hatakeyama ; Erika S. Nishiduka [4] ; Alexandre K. Tashima [5] ; Sávio S. Sant’Anna ; Sávio S. SantAnna [6] ; Kathleen F. Grego [7] ; Karen de Morais-Zani ; Anita M. Tanaka-Azevedo
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 26, p. -, 2020.
Citações Web of Science: 0
Resumo

Abstract Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy. Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and -negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected. Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom. (AU)

Processo FAPESP: 17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
Beneficiário:Anita Mitico Tanaka-Azevedo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/11108-0 - Estudo comparativo da composição das biomoléculas dos venenos das serpentes Bothrops jararaca e Crotalus durissus terrificus nascidas e mantidas no Instituto Butantan e recém-chegadas da natureza
Beneficiário:Karen de Morais Zani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/20106-9 - Peptidômica dos venenos de serpentes e aranhas brasileiras
Beneficiário:Alexandre Keiji Tashima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
Beneficiário:Karen de Morais Zani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/03311-5 - Estudo da presença da crotamina no veneno das serpentes Crotalus durissus terrificus, Crotalus durissus collilineatus e Crotalus durissus cascavella do plantel e recém-chegadas no Instituto Butantan
Beneficiário:Lidia Jorge Tasima
Linha de fomento: Bolsas no Brasil - Iniciação Científica