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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature

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Lídia J. Tasima ; Caroline Serino-Silva ; Daniela M. Hatakeyama ; Erika S. Nishiduka ; Alexandre K. Tashima ; Sávio S. Sant’Anna ; Kathleen F. Grego ; Karen de Morais-Zani ; Anita M. Tanaka-Azevedo
Total Authors: 9
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 26, p. -, 2020.
Web of Science Citations: 0

Abstract Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy. Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and -negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected. Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom. (AU)

FAPESP's process: 17/01890-0 - Comparative study of Crotalus durissus terrificus snake venoms born in captivity in the Laboratory of Herpetology of Butantan Institute and the national crotalic reference venom
Grantee:Anita Mitico Tanaka-Azevedo
Support type: Regular Research Grants
FAPESP's process: 16/03311-5 - Study of the presence of crotamine in Crotalus durissus terrificus, Crotalus durissus collilineatus and Crotalus durissus cascavella snake venom of squad and received in the Instituto Butantan
Grantee:Lidia Jorge Tasima
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/11108-0 - Comparative study of biomolecules composition of Bothrops jararaca and Crotalus durissus terrificus venom born and maintained at the Butantan Institute and newly arrived from nature
Grantee:Karen de Morais Zani
Support type: Regular Research Grants
FAPESP's process: 17/20106-9 - Peptidomics of Brazilian snake and spider venoms
Grantee:Alexandre Keiji Tashima
Support type: Regular Research Grants
FAPESP's process: 17/16908-2 - Proteomic characterization and enzymatic and pathophysiological activities of the venom of the snake species that compose the Bothrops neuwiedi group
Grantee:Karen de Morais Zani
Support type: Regular Research Grants