Comparative study of Crotalus durissus terrificus snake venoms born in captivity in the Laboratory of Herpetology of Butantan Institute and the national crotalic reference venom

• Grant number: 17/01890-0
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2017 - April 30, 2019
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Anita Mitico Tanaka-Azevedo
• Grantee: Anita Mitico Tanaka-Azevedo
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Aparecida Sadae Tanaka ; Karen de Morais Zani ; Kathleen Fernandes Grego ; Sávio Stefanini Sant Anna

Abstract

Snakes of the genus Crotalus (Viperidae family) are very important from the epidemiological point of view, once, although they account only for about 9% of ophidian accidents, they are with the highest lethality (1.1%). In Brazil, the genus Crotalus is represented by a single species, Crotalus durissus (C.d.), which has a wide geographical distribution in Brazil and has 5 subspecies, C.d.marajoensis, C.d.ruruima, C.d.collillineatus, C.d.cascavella and C.d. terrificus, the latter being more legendary and widely dispersed in southern Brazil. Crotalic venom can cause neuro and myotoxicity. In addition, symptoms like systemic neurotoxicity accompanied by rhabdomyolysis result in tubular necrosis and renal failure, common in crotalic poisoning, thus constituting the leading cause of death. All these symptoms are mainly caused by crotoxin. Besides crotoxin, gyroxine, crotamine and convulxine are proteins found in significant amounts and variable in crotalic venom. The elucidation of these activities is important both for envenomation-related research and for the production of anti-crotalic sera, considering that, if present, variations may result in the production of less effective sera and inconsistent results in scientific research. In this context, although there is a search for standardization and stabilization of the different components of ophidian venoms, they still lack conclusive results in both aspects.It is clear the necessity of the use of reference venoms at the national level, as well as the standardization of the methods of testing the biological activities of the Brazilian venom of snakes to ensure a uniformity in antiofidic sera. Thus, from 1987, the National Institute of Quality Control in Health (INCQS) and the serum producers' Brazilian laboratories began to use the National Reference Crotalic Venom, in order to minimize interlaboratory differences in the determinations of potency of this antivenom. Consequently, the Butantan Institute became responsible for the preparation and distribution of National Reference Crotalic Venom. For the preparation of the reference venoms, the first extractions of snakes that have just arrived at the Butantan Institute are used. Since then, 02 batches of National Reference Crotalic Venom have been produced from the venom of the snake C. d. terrificus, whose potency was determined exclusively by INCQS, which is responsible for its storage and distribution.Considering the importance of elucidating the composition of both reference and venomous venoms, this study intends to compare the venom of the National Reference Snake Crotalus durissus terrificus with those of the snakes born and kept in captivity in the Laboratory of Herpetology of the Butantan Institute. It is important to emphasize the importance of this work, since, due to the reduction in the supply of these animals from the nature, there may be a scarcity of these animals for the production of the Crotalic Snake National Reference, thus the use of the venoms of the captive snakes would supply this demand. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; GALIZIO, NATHALIA DA COSTA; TASIMA, LIDIA JORGE; PAGOTTO, JOSIAS FALARARO; TEIXEIRA DA ROCHA, MARISA MARIA; MARCELINO, JOSE ROBERTO; SANT'ANNA, SAVIO STEFANINI; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; et al. Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?. JOURNAL OF PROTEOMICS, v. 174, p. 17-27, MAR 1 2018. (14/11108-0, 16/09976-9, 17/01890-0, 12/19321-9)

DE FARIAS, IASMIM BAPTISTA; DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO S.; DA ROCHA, MARISA M. T.; GREGO, KATHLEEN F.; ANDRADE-SILVA, DEBORA; SERRANO, SOLANGE M. T.; TANAKA-AZEVEDO, ANITA M.. Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom. JOURNAL OF PROTEOMICS, v. 174, p. 36-46, MAR 1 2018. (13/07467-1, 16/16935-7, 14/11108-0, 17/01890-0, 17/16908-2, 13/05357-4)

GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; STUGINSKI, DANIEL RODRIGUES; ESTIMA ABREU, PATRICIA ANTONIA; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; DE MORAIS-ZANI, KAREN. Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes. JOURNAL OF PROTEOMICS, v. 186, p. 56-70, AUG 30 2018. (14/11108-0, 16/09976-9, 17/01890-0, 17/16908-2)

LEANDRO NORBERTO DA SILVA-JÚNIOR; LARA DE SOUZA ABREU; CAROLINE FABRI BITTENCOURT RODRIGUES; NATHÁLIA DA COSTA GALIZIO; WESLEI DA SILVA AGUIAR; CAROLINE SERINO-SILVA; VALDOMIRO SOUZA DOS SANTOS; ISABELLA ALVES COSTA; LUIS VICENTE FRANCO OLIVEIRA; SÁVIO STEFANINI SANTANNA; et al. Geographic variation of individual venom profile of Crotalus durissus snakes. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-08-26. (14/11108-0, 16/09976-9, 17/01890-0, 18/25786-0, 18/14724-4, 17/16908-2)

SILVA, CAROLINE SERINO; BITTENCOURT RODRIGUES, CAROLINE FABRI; GREGO, KATHLEEN FERNANDES; DE MORAIS-ZAN, KAREN; TANAKA-AZEVEDO, ANITA MITICO. NEUTRALIZATION OF THE LOCAL EFFECTS OF SNAKE VENOM PLA2 BY BOTHROPS JARARACA gamma PHOSPHOLIPASE INHIBITOR (gamma BJPLI). Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 14/11108-0, 17/01890-0, 13/05357-4)

KAVAZOI, VICTOR KOITI; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GREGO, KATHLEEN FERNANDES; MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. IDENTIFICATION AND PURIFICATION OF PHOSPHOLIPASE A2 INHIBITORS (PLIS) FROM SNAKE BLOOD MAINTAINED IN THE LABORATORY OF HERPETOLOGY OF BUTANTAN INSTITUTE. Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 17/17165-3, 17/01890-0)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; GREGO, KATHLEEN FERNANDES; SANT'ANNA, SAVIO STEFANINI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. BOTHROPS JARARACUSSU ONTOGENETIC AND SEASONAL VENOM VARIABILITY: WHAT WE GOT SO FAR. Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 18/20651-0, 17/01890-0)

VENANCIO DE LIMA, EDUARDO OLIVEIRA; TASIMA, LIDIA JORGE; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GALIZIO, NATHALIA DA COSTA; CHIARELLI, TASSIA; NISHIDUKA, ERIKA SAYURI; TEIXEIRA DA ROCHA, MARISA MARIA; SANT'ANNA, SAVIO STEFANINI; et al. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, v. 193, p. 73-83, APR 15 2021. (18/25786-0, 14/11108-0, 17/01890-0, 16/09976-9, 17/20106-9, 17/21052-0, 17/16908-2, 18/14724-4)

LÍDIA J. TASIMA; CAROLINE SERINO-SILVA; DANIELA M. HATAKEYAMA; ERIKA S. NISHIDUKA; ALEXANDRE K. TASHIMA; SÁVIO S. SANTANNA; KATHLEEN F. GREGO; KAREN DE MORAIS-ZANI; ANITA M. TANAKA-AZEVEDO. Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-04-06. (14/11108-0, 16/03311-5, 17/01890-0, 17/20106-9, 17/16908-2)

PREZOTO, B. C.; TANAKA-AZEVEDO, A. M.; MARCELINO, J. R.; TASHIMA, A. K.; NISHIDUKA, E. S.; KAPRONEZAI, J.; MOTA, J. O.; ROCHA, M. M. T.; SERINO-SILVA, C.; OGUIURA, N.. A functional and thromboelastometric-based micromethod for assessing crotoxin anticoagulant activity and antiserum relative potency against Crotalus durissus terrificus venom. Toxicon, v. 148, p. 26-32, JUN 15 2018. (17/01890-0)

SERINO-SILVA, CAROLINE; MORAIS-ZANI, KAREN; TOYAMA, MARCOS HIKARI; TOYAMA, DANIELA DE OLIVEIRA; GAETA, HENRIQUE HESSE; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; TASHIMA, ALEXANDRE KEIJI; GREGO, KATHLEEN FERNANDES; TANAKA-AZEVEDO, ANITA MITICO. Purification and characterization of the first gamma-phospholipase inhibitor (gamma PLI) from Bothrops jararaca snake serum. PLoS One, v. 13, n. 3, MAR 5 2018. (14/11108-0, 13/12826-0, 17/01890-0, 17/16908-2, 12/19321-9, 13/05357-4)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations. PLoS One, v. 14, n. 9, SEP 12 2019. (17/01890-0, 17/16908-2, 14/11108-0, 17/20106-9, 18/20651-0, 18/14724-4, 18/25786-0)

HATAKEYAMA, DANIELA MIKI; TASIMA, LIDIA JORGE; BRAVO-TOBAR, CESAR ADOLFO; SERINO-SILVA, CAROLINE; TASHIMA, ALEXANDRE KEIJI; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; VENANCIO DE LIMA, EDUARDO OLIVEIRA; KAVAZOI, VICTOR KOITI; et al. Venom complexity of Bothrops atrox (common lancehead) siblings. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, OCT 12 2020. (17/16908-2, 18/25786-0, 17/01890-0, 17/20106-9)

TASIMA, LIDIA J.; HATAKEYAMA, DANIELA M.; SERINO-SILVA, CAROLINE; RODRIGUES, CAROLINE F. B.; DE LIMA, V, EDUARDO O.; SANT'ANNA, SAVIO S.; GREGO, KATHLEEN F.; DE MORAIS-ZANI, KAREN; SANZ, LIBIA; CALVETE, JUAN J.; et al. Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom. Toxicon, v. 185, p. 26-35, OCT 15 2020. (17/01890-0, 17/16908-2, 18/25899-0, 17/26533-6, 18/25786-0)