Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes

Author(s)

Galizio, Nathalia da Costa ^[1] ; Serino-Silva, Caroline ^{[1, 2]} ; Stuginski, Daniel Rodrigues ^[1] ; Estima Abreu, Patricia Antonia ^[3] ; Sant'Anna, Savio Stefanini ^[1] ; Grego, Kathleen Fernandes ^[1] ; Tashima, Alexandre Keiji ^[4] ; Tanaka-Azevedo, Anita Mitico ^{[1, 2]} ; de Morais-Zani, Karen ^{[1, 2]}

Affiliation

^[1] Inst Butantan, Lab Herpetol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Interunidades Biotecnol, Sao Paulo - Brazil

^[3] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF PROTEOMICS; v. 186, p. 56-70, AUG 30 2018.
• Web of Science Citations: 3

Abstract

Intraspecific venom variability has been extensively reported in a number of species and is documented to be the result of several factors. However, current evidence for snake venom variability related to captivity maintenance is controversial. Here we report a compositional and functional investigation of individual and pooled venoms from long-term captive (LTC) and recently wild-caught (RWC) B. jararaca snakes. The composition of individual venoms showed a remarkable variability in terms of relative abundance of toxins (evidenced by 1-DE and RPHPLC), enzymatic activities (proteolytic, PLA(2), and LAAO) and coagulant activity, even among captive specimens. Thus, no compositional and functional pattern could be established to assign each individual venom to a specific group. Conversely, pooled venom from LTC and RWC snakes showed no significant differences regarding protein composition (characterized by 1-DE and shotgun proteomics), enzymatic activities (proteolytic, PLA2 and LAAO) and biological function (coagulant, hemorrhagic and lethal activities), except for edematogenic activity, which was more prominent in RWC venom pool. Additionally, both pooled venoms displayed similar immunoreactivity with the bothropic antivenom produced by Instituto Butantan. Taken together, our results highlight the complexity and the high intraspecific variation of B. jararaca venom, that is not influenced at a discernible extent by captivity maintenance. (AU)

• FAPESP's process: 17/16908-2 - Proteomic characterization and enzymatic and pathophysiological activities of the venom of the snake species that compose the Bothrops neuwiedi group
• Grantee: Karen de Morais Zani
• Support type: Regular Research Grants
• FAPESP's process: 16/09976-9 - Comparison of venom from Bothrops jararaca snake maintened in captivity and newly arrived from nature
• Grantee: Nathália da Costa Galizio
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/01890-0 - Comparative study of Crotalus durissus terrificus snake venoms born in captivity in the Laboratory of Herpetology of Butantan Institute and the national crotalic reference venom
• Grantee: Anita Mitico Tanaka-Azevedo
• Support type: Regular Research Grants
• FAPESP's process: 14/11108-0 - Comparative study of biomolecules composition of Bothrops jararaca and Crotalus durissus terrificus venom born and maintained at the Butantan Institute and newly arrived from nature
• Grantee: Karen de Morais Zani
• Support type: Regular Research Grants