Comparative study of biomolecules composition of Bothrops jararaca and Crotalus durissus terrificus venom born and maintained at the Butantan Institute and newly arrived from nature

• Grant number: 14/11108-0
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2014 - May 31, 2017
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Karen de Morais Zani
• Grantee: Karen de Morais Zani
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Anita Mitico Tanaka-Azevedo ; Aparecida Sadae Tanaka ; Kathleen Fernandes Grego ; Marisa Maria Teixeira da Rocha ; Sávio Stefanini Sant Anna

Abstract

Snake venoms are the most studied animal toxins. The variability of venom composition was described not only intra- and interspecies but also according to sex, age and geographical distribution of the snakes, and in most studies the diet of snakes was considered as the triggering factor for this variation. The Herpetology Laboratory of the Butantan Institute provides venoms for antivenom production and for scientific research not only for the Institute, but to other institutions as well. Currently, it is observed that the number of snakes donated to the Institute has been steadily decreasing. This fact has stimulated the Herpetology Laboratory, where the snakes are kept, to develop technics to reproduce snakes in captivity and to become self-sufficient in creating these animals. Thus, the snake's colony of Herpetology Laboratory is composed mostly by animals born and/or kept in captivity for a long period of time and fed exclusively with mice and/or rats. Considering that the presence of changes in venom composition of snakes kept in captivity has not yet been investigated, the aim of this study is to evaluate the influence of diet on composition of B. jararaca and C. durissus terrificus snakes maintained in captivity in the Herpetology Laboratory of Butantan Institute. This evaluation involves the biochemical characterization of the venoms using classical methodology, such as protein measurement, polyacrylamide gel electrophoresis, including the identification of the proteins of interest by mass spectrometry, and high performance liquid chromatography. This study also includes the biological characterization of the samples, which comprise not only the determination of their well established activities, as proteolytic, coagulant and hemorrhagic, and the determination of lethal dose, but also "antivenomics" analysis and evaluation of the genotoxicity of toxins, activity which are attributed to the late manifestations of envenoming, through comet and micronucleus analyzes. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO BRAGA, JACQUELINE RAMOS; DE MORAIS-ZANI, KAREN; PEREIRA, DIEGO DOS SANTOS; SANT'ANNA, SAVIO STEFANINI; GALIZIO, NATHALIA DA COSTA; TANAKA-AZEVEDO, ANITA MITICO; GOMES VILARINHO, ARIEL RODRIGUES; RODRIGUES, JOSE LUCCA; TEIXEIRA DA ROCHA, MARISA MARIA. Sexual and ontogenetic variation of Bothrops leucurus venom. Toxicon, v. 184, p. 127-135, SEP 2020. (14/11108-0, 18/14724-4, 17/16908-2, 16/09976-9, 18/25786-0)

LÍDIA J. TASIMA; CAROLINE SERINO-SILVA; DANIELA M. HATAKEYAMA; ERIKA S. NISHIDUKA; ALEXANDRE K. TASHIMA; SÁVIO S. SANTANNA; KATHLEEN F. GREGO; KAREN DE MORAIS-ZANI; ANITA M. TANAKA-AZEVEDO. Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-04-06. (17/01890-0, 17/16908-2, 14/11108-0, 16/03311-5, 17/20106-9)

HATAKEYAMA, DANIELA M. `; DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; GREGO, KATHLEEN F.; SANT'ANNA, SAVIO S.; FERNANDES, WILSON; ANIZ, PATRICIA A. E. A.; TORQUATO, RICARDO J. S.; TANAKA, APARECIDA S.; SANZ, LIBIA; et al. Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storage. Toxicon, v. 141, p. 34-42, JAN 2018. (12/10266-5, 14/11108-0, 13/00267-7, 12/12997-7, 14/50510-8, 13/05357-4)

GOMES, CICERA MARIA; DE MORAIS-ZANI, KAREN; LU, STEPHEN; BUARQUE, DIEGO DE SOUZA; CARDOSO BRAZ, GLORIA REGINA; GREGO, KATHLEEN FERNANDES; TANAKA, APARECIDA SADAE; TANAKA-AZEVEDO, ANITA MITICO. Differential transcript profile of inhibitors with potential anti-venom role in the liver of juvenile and adult Bothrops jararaca snake. PeerJ, v. 5, APR 27 2017. (12/03657-8, 14/11108-0, 13/05357-4)

SERINO-SILVA, CAROLINE; MORAIS-ZANI, KAREN; TOYAMA, MARCOS HIKARI; TOYAMA, DANIELA DE OLIVEIRA; GAETA, HENRIQUE HESSE; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; TASHIMA, ALEXANDRE KEIJI; GREGO, KATHLEEN FERNANDES; TANAKA-AZEVEDO, ANITA MITICO. Purification and characterization of the first gamma-phospholipase inhibitor (gamma PLI) from Bothrops jararaca snake serum. PLoS One, v. 13, n. 3, MAR 5 2018. (14/11108-0, 13/12826-0, 17/01890-0, 17/16908-2, 12/19321-9, 13/05357-4)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations. PLoS One, v. 14, n. 9, SEP 12 2019. (17/01890-0, 17/16908-2, 14/11108-0, 17/20106-9, 18/20651-0, 18/14724-4, 18/25786-0)

DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; GALIZIO, NATHALIA DA COSTA; TASIMA, LIDIA JORGE; PAGOTTO, JOSIAS FALARARO; TEIXEIRA DA ROCHA, MARISA MARIA; MARCELINO, JOSE ROBERTO; SANT'ANNA, SAVIO STEFANINI; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; et al. Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?. JOURNAL OF PROTEOMICS, v. 174, p. 17-27, MAR 1 2018. (14/11108-0, 16/09976-9, 17/01890-0, 12/19321-9)

DE FARIAS, IASMIM BAPTISTA; DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO S.; DA ROCHA, MARISA M. T.; GREGO, KATHLEEN F.; ANDRADE-SILVA, DEBORA; SERRANO, SOLANGE M. T.; TANAKA-AZEVEDO, ANITA M.. Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom. JOURNAL OF PROTEOMICS, v. 174, p. 36-46, MAR 1 2018. (13/07467-1, 16/16935-7, 14/11108-0, 17/01890-0, 17/16908-2, 13/05357-4)

SILVA, CAROLINE SERINO; BITTENCOURT RODRIGUES, CAROLINE FABRI; GREGO, KATHLEEN FERNANDES; DE MORAIS-ZAN, KAREN; TANAKA-AZEVEDO, ANITA MITICO. NEUTRALIZATION OF THE LOCAL EFFECTS OF SNAKE VENOM PLA2 BY BOTHROPS JARARACA gamma PHOSPHOLIPASE INHIBITOR (gamma BJPLI). Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 14/11108-0, 17/01890-0, 13/05357-4)

LEANDRO NORBERTO DA SILVA-JÚNIOR; LARA DE SOUZA ABREU; CAROLINE FABRI BITTENCOURT RODRIGUES; NATHÁLIA DA COSTA GALIZIO; WESLEI DA SILVA AGUIAR; CAROLINE SERINO-SILVA; VALDOMIRO SOUZA DOS SANTOS; ISABELLA ALVES COSTA; LUIS VICENTE FRANCO OLIVEIRA; SÁVIO STEFANINI SANTANNA; et al. Geographic variation of individual venom profile of Crotalus durissus snakes. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-08-26. (17/16908-2, 16/09976-9, 18/25786-0, 17/01890-0, 14/11108-0, 18/14724-4)

GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; STUGINSKI, DANIEL RODRIGUES; ESTIMA ABREU, PATRICIA ANTONIA; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; DE MORAIS-ZANI, KAREN. Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes. JOURNAL OF PROTEOMICS, v. 186, p. 56-70, AUG 30 2018. (14/11108-0, 16/09976-9, 17/01890-0, 17/16908-2)

VENANCIO DE LIMA, EDUARDO OLIVEIRA; TASIMA, LIDIA JORGE; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GALIZIO, NATHALIA DA COSTA; CHIARELLI, TASSIA; NISHIDUKA, ERIKA SAYURI; TEIXEIRA DA ROCHA, MARISA MARIA; SANT'ANNA, SAVIO STEFANINI; et al. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, v. 193, p. 73-83, APR 15 2021. (18/25786-0, 14/11108-0, 17/01890-0, 16/09976-9, 17/20106-9, 17/21052-0, 17/16908-2, 18/14724-4)