NLRC4 inflammasome has a protective role on inflammatory bone resorption in a murine model of periodontal disease

Autor(es)

Rocha, Fernanda R. G. ^{[1, 2]} ; Delitto, Andrea E. ^[3] ; Chaves de Souza, Joao A. ^[4] ; Maldonado, Laura A. G. ^[2] ; Wallet, Shannon M. ^[5] ; Rossa Jr, Carlos

Afiliação do(s) autor(es)

^[1] Univ Florida, Coll Dent, Dept Oral Biol, Gainesville, FL 32610 - USA

^[2] UNESP State Univ Sao Paulo, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP - Brazil

^[3] Univ Florida, Hlth Sci Ctr, Dept Phys Therapy, Gainesville, FL - USA

^[4] Fed Univ Goias UFG, Sch Dent, Dept Stomatol, Goiania, Go - Brazil

^[5] East Carolina Univ, Coll Dent Med, Dept Foundat Sci, Greenville, NC 27858 - USA

• Tipo de documento: Artigo Científico
• Fonte: Immunobiology; v. 225, n. 1 JAN 2020.
• Citações Web of Science: 0

Resumo

There is virtually no information on the role of NLRC4 inflammasome on bone resorption and inflammation associated with periodontitis. Bacterial-associated experimental periodontitis was induced in wild-type (WT) and Nlrc4-KO C57BL/6 mice. 3 mu L of a 1 x 10(9) UFC/mL PBS suspension of heat-killed Gram-negative bacteria were injected (3x/week for 4 weeks) directly into the gingival tissues of WT and Nlrc4-KO mice (n = 6/genotype). Control animals were injected bilaterally (3x/week for 4 weeks) in the same sites with the same volume of the PBS vehicle. Alveolar bone resorption was quantified by mu CT. Inflammatory infiltrate in the gingival tissues was assessed qualitatively in H\&E-stained slides and by the detection of a pan-leukocyte marker (CD45) and a neutrophil marker (Ly6G) using immunofluorescence. Modulation of Rankl, Mmp-13, Tnf-a, Il-6 and Il-10 expression in the gingival tissues was determined by RT-qPCR. Osteoclastogenesis was assessed in vivo by biochemical staining for TRAP. The relevance of NLRC4 for RANKL-induced osteoclastic differentiation and activity was investigated in vitro using bone marrow-derived macrophages from WT and Nlrc4-KO mice. Bone resorption was significantly greater in Nlrc4-KO mice; however there were no differences between WT and Nlrc4-KO mice on osteoclast numbers and on the inflammatory infiltrate. In vitro, osteoclast activity was significantly enhanced in Nlrc4-deficient macrophages; whereas RANKL-induced differentiation was not affected. Expression of the selected candidate genes was also similarly increased by the induction of experimental periodontal disease, except for the expression of Tnf-alpha and Il-10, which was already significantly higher in the gingival tissues of Nlrc4-KO mice. We conclude that NLRC4 inflammasome has a protective role on inflammatory bone resorption in this experimental model. Furthermore, the bone-sparing effect may be related with the modulation of osteoclast activity. (AU)

• Processo FAPESP: 14/17544-6 - O inflamassomo NLRC4 e a resposta imune e perda óssea na doença periodontal experimental
• Beneficiário: Fernanda Regina Godoy Rocha
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/04926-8 - Inflamassomos NLRC4 e NLRP3 na doença periodontal experimental induzida por Aggregatibacter actinomycetemcomitans
• Beneficiário: Fernanda Regina Godoy Rocha
• Modalidade de apoio: Bolsas no Brasil - Doutorado