Effect of ovariectomy on maxilla and mandible dimensions of female rats

Autor(es):Mostrar menos -

Omori, Marjorie Ayumi ^[1] ; Maranon-Vasquez, Guido Artemio ^[2] ; Romualdo, Priscilla Coutinho ^[1] ; Neto, Evandro Carneiro Martins ^[3] ; Stuani, Maria Bernadete Sasso ^[1] ; Matsumoto, Mirian Aiko Nakane ^[1] ; Nelson-Filho, Paulo ^[1] ; Proff, Peter ^[4] ; Leon, Jorge Esquiche ^[5] ; Kirschneck, Christian ^[4] ; Kuchler, Erika C. ^{[6, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N, Campus USP, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Sch Dent, Dept Pediat Dent & Orthodont, Rio De Janeiro - Brazil

^[3] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodont, Ribeirao Preto - Brazil

^[4] Univ Med Ctr Regensburg, Dept Orthodont, Regensburg - Germany

^[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Stomatol Publ Hlth & Forens Dent, Ribeirao Preto - Brazil

^[6] Univ Posit, Dept Dent, Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORTHODONTICS & CRANIOFACIAL RESEARCH; v. 23, n. 3 APR 2020.
• Citações Web of Science: 0

Resumo

Objective The role of oestrogen in craniofacial growth still remains unclear. Therefore, the present study aimed to assess the effect of oestrogen deficiency on maxilla and mandible dimensions. Setting and Sample Population The study was conducted at the Department of Pediatric Dentistry at the School of Dentistry of Ribeirao Preto, University of Sao Paulo, and used forty female Wistar rats. Material and Methods Ovariectomy (OVX) and placebo surgery (Sham) were performed when animals were twenty-one days old (prepubertal stage). Dimensions of the maxilla and mandible were assessed by craniometric analysis using radiographs, during and after puberty of the animals (45 and 63 days old, respectively). Quantitative real-time PCR and immunohistochemical analyses were performed to determine the expression and localization, respectively, of oestrogen receptor alpha (ER alpha) and oestrogen receptor beta (ER beta) in different growth sites of the evaluated structures at puberty. The differences between the groups for each outcome were evaluated using the t test with an established alpha error of 5%. Results There were significant differences between the OVX and Sham groups for horizontal and vertical linear measurements in the maxilla and the mandible at both pubertal and post-pubertal stages (P < .05). The ovariectomized rats showed significantly greater measures for all dimensions assessed. No differences in gene expression of ER alpha and ER beta were identified at the different growth sites between the OVX and Sham groups (P > .05). Immunohistochemical analyses revealed the presence of both oestrogen receptors in osteoblasts and chondrocytes in the midpalatal suture and mandibular condyle, respectively, in the OVX and Sham groups. Conclusion Our results suggest that oestrogen deficiency from the prepubertal stage might increase the growth of the maxilla and mandible in female rats. (AU)

• Processo FAPESP: 16/08149-1 - Avaliação do papel do estrógeno nos tecidos dentofaciais
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 16/13982-4 - Avaliação do papel do estrógeno no crescimento e desenvolvimento da maxila e da mandíbula
• Beneficiário: Marjorie Ayumi Omori
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/06866-5 - Avaliação do papel do estrógeno no desenvolvimento dentofacial
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/16990-0 - Associação de variações genéticas com alterações esqueléticas dos arcos dentários
• Beneficiário: Guido Artemio Maranon Vasquez
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico