Effect of ovariectomy on maxilla and mandible dimensions of female rats

Author(s):Show less -

Omori, Marjorie Ayumi ^[1] ; Maranon-Vasquez, Guido Artemio ^[2] ; Romualdo, Priscilla Coutinho ^[1] ; Neto, Evandro Carneiro Martins ^[3] ; Stuani, Maria Bernadete Sasso ^[1] ; Matsumoto, Mirian Aiko Nakane ^[1] ; Nelson-Filho, Paulo ^[1] ; Proff, Peter ^[4] ; Leon, Jorge Esquiche ^[5] ; Kirschneck, Christian ^[4] ; Kuchler, Erika C. ^{[6, 1]}

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N, Campus USP, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Sch Dent, Dept Pediat Dent & Orthodont, Rio De Janeiro - Brazil

^[3] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodont, Ribeirao Preto - Brazil

^[4] Univ Med Ctr Regensburg, Dept Orthodont, Regensburg - Germany

^[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Stomatol Publ Hlth & Forens Dent, Ribeirao Preto - Brazil

^[6] Univ Posit, Dept Dent, Curitiba, Parana - Brazil

• Document type: Journal article
• Source: ORTHODONTICS & CRANIOFACIAL RESEARCH; v. 23, n. 3 APR 2020.
• Web of Science Citations: 0

Abstract

Objective The role of oestrogen in craniofacial growth still remains unclear. Therefore, the present study aimed to assess the effect of oestrogen deficiency on maxilla and mandible dimensions. Setting and Sample Population The study was conducted at the Department of Pediatric Dentistry at the School of Dentistry of Ribeirao Preto, University of Sao Paulo, and used forty female Wistar rats. Material and Methods Ovariectomy (OVX) and placebo surgery (Sham) were performed when animals were twenty-one days old (prepubertal stage). Dimensions of the maxilla and mandible were assessed by craniometric analysis using radiographs, during and after puberty of the animals (45 and 63 days old, respectively). Quantitative real-time PCR and immunohistochemical analyses were performed to determine the expression and localization, respectively, of oestrogen receptor alpha (ER alpha) and oestrogen receptor beta (ER beta) in different growth sites of the evaluated structures at puberty. The differences between the groups for each outcome were evaluated using the t test with an established alpha error of 5%. Results There were significant differences between the OVX and Sham groups for horizontal and vertical linear measurements in the maxilla and the mandible at both pubertal and post-pubertal stages (P < .05). The ovariectomized rats showed significantly greater measures for all dimensions assessed. No differences in gene expression of ER alpha and ER beta were identified at the different growth sites between the OVX and Sham groups (P > .05). Immunohistochemical analyses revealed the presence of both oestrogen receptors in osteoblasts and chondrocytes in the midpalatal suture and mandibular condyle, respectively, in the OVX and Sham groups. Conclusion Our results suggest that oestrogen deficiency from the prepubertal stage might increase the growth of the maxilla and mandible in female rats. (AU)

• FAPESP's process: 16/08149-1 - Evaluation of the role of estrogen in dentofacial tissues
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 16/13982-4 - Evaluation of the role of estrogen in the growth and development of the maxilla and mandible
• Grantee: Marjorie Ayumi Omori
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Research Grants - Young Investigators Grants