Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine

Autor(es)

Gomes de Melo, Bruna Alice ^[1] ; Franca, Carla Giometti ^[1] ; Davila, Jose Luis ^[2] ; Batista, Nilza Alzira ^[3] ; Caliari-Oliveira, Carolina ^[4] ; d'Avila, Marcos Akira ^[2] ; Malheiros Luzo, Angela Cristina ^[5] ; Santos Duarte Lana, Jose Fabio ^[6] ; Andrade Santana, Maria Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Sch Mech Engn, Dept Mfg & Mat Engn, BR-13083860 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Orthopaed Biomat Lab, BR-13083887 Campinas, SP - Brazil

^[4] Supera Innovat & Technol Pk, In Situ Cell Therapy, BR-14056680 Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, Haematol & Hemotherapy Ctr, Umbil Cord Blood Bank, BR-13083878 Campinas, SP - Brazil

^[6] Bone & Cartilage Inst, BR-13334170 Indaiatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 109, APR 2020.
• Citações Web of Science: 1

Resumo

Autologous leukocyte- and platelet-rich plasma (L-PRP) combined with hyaluronic acid (HA) has been widely used in local applications for cartilage and bone regeneration. The association between L-PRP and HA confers structural and rheological changes that differ among individual biomaterials but has not been investigated. Therefore, the standardization and characterization of L-PRP-HA are important to consider when comparing performance results to improve future clinical applications. To this end, we prepared semi-interpenetrating polymer networks (semi-IPNs) of L-PRP and HA and characterized their polymerization kinetics, morphology, swelling ratio, stability and rheological behavior, which we found to be tunable according to the HA molar mass (MM). Mesenchymal stem cells derived from human adipose tissue (h-AdMSCs) seeded in the semi-IPNs had superior viability and chondrogenesis and osteogenesis capabilities compared to the viability and capabilities of fibrin. We have demonstrated that the preparation of the semi-IPNs under controlled mixing ensured the formation of cell-friendly hydrogels rich in soluble factors and with tunable properties according to the HA MM, rendering them suitable for clinical applications in regenerative medicine. (AU)

• Processo FAPESP: 15/23134-8 - Influência da associação entre plasma-rico em plaquetas e ácido hialurônico na liberação de fatores de crescimento, proliferação celular e diferenciação osteogênica/condrogênica
• Beneficiário: Bruna Alice Gomes de Melo
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/10132-0 - Plasma-rico em plaquetas associado ao ácido hialurônico para aplicações em ortopedia regenerativa: do desenvolvimento tecnológico das preparações às caracterizações físico-químicas e biológicas in vitro
• Beneficiário: Maria Helena Andrade Santana
• Linha de fomento: Auxílio à Pesquisa - Regular