Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine

Author(s)

Gomes de Melo, Bruna Alice ^[1] ; Franca, Carla Giometti ^[1] ; Davila, Jose Luis ^[2] ; Batista, Nilza Alzira ^[3] ; Caliari-Oliveira, Carolina ^[4] ; d'Avila, Marcos Akira ^[2] ; Malheiros Luzo, Angela Cristina ^[5] ; Santos Duarte Lana, Jose Fabio ^[6] ; Andrade Santana, Maria Helena ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Sch Mech Engn, Dept Mfg & Mat Engn, BR-13083860 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Orthopaed Biomat Lab, BR-13083887 Campinas, SP - Brazil

^[4] Supera Innovat & Technol Pk, In Situ Cell Therapy, BR-14056680 Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, Haematol & Hemotherapy Ctr, Umbil Cord Blood Bank, BR-13083878 Campinas, SP - Brazil

^[6] Bone & Cartilage Inst, BR-13334170 Indaiatuba, SP - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 109, APR 2020.
• Web of Science Citations: 1

Abstract

Autologous leukocyte- and platelet-rich plasma (L-PRP) combined with hyaluronic acid (HA) has been widely used in local applications for cartilage and bone regeneration. The association between L-PRP and HA confers structural and rheological changes that differ among individual biomaterials but has not been investigated. Therefore, the standardization and characterization of L-PRP-HA are important to consider when comparing performance results to improve future clinical applications. To this end, we prepared semi-interpenetrating polymer networks (semi-IPNs) of L-PRP and HA and characterized their polymerization kinetics, morphology, swelling ratio, stability and rheological behavior, which we found to be tunable according to the HA molar mass (MM). Mesenchymal stem cells derived from human adipose tissue (h-AdMSCs) seeded in the semi-IPNs had superior viability and chondrogenesis and osteogenesis capabilities compared to the viability and capabilities of fibrin. We have demonstrated that the preparation of the semi-IPNs under controlled mixing ensured the formation of cell-friendly hydrogels rich in soluble factors and with tunable properties according to the HA MM, rendering them suitable for clinical applications in regenerative medicine. (AU)

• FAPESP's process: 15/23134-8 - Influence of the association between platelet-rich plasma and hyaluronic acid on the release of growth factors, cellular proliferation and osteogenic/ chondrogenic differentiation
• Grantee: Bruna Alice Gomes de Melo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/10132-0 - Platelet-rich plasma associated to hyaluronic acid for applications in regenerative orthopedics: from the technological development of preparations to physicochemical and in vitro biological characterizations
• Grantee: Maria Helena Andrade Santana
• Support Opportunities: Regular Research Grants