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Platelet-rich plasma associated to hyaluronic acid for applications in regenerative orthopedics: from the technological development of preparations to physicochemical and in vitro biological characterizations


Platelet-rich plasma (PRP) is an autologous product prepared from whole blood with high capability to stimulate tissue regeneration. Hyaluronic acid (HA) is the main component of synovial fluid, which has multiple functions in viscossupplementation and cell signalization. In the last five years, the association of free HA and PRP has been produced clinical benefits to regenerative orthopedics. However, fundamental studies are still scarce for characterization of this association. Since 2009, the proponent group of this project, in an interdisciplinary association with the medical area, has been dedicated to the studies of basic science of PRP, and also of structural modifications of HA and its association of PRP. In this context, this project aims to broaden the spectrum of these research with the following studies: influence of the balance between platelets and leukocytes in PRP (L-PRP type) and optimization of the conditions of their preparation by centrifugation aiming standardization; influence of HA concentration gradient in centrifugation on the preparation of L-PRP; effects of the molecular weight (106, 105 and 103Da) of the free HA on proliferation of human-adipose mesenchymal cells (h-AdMSCs) and capability for differentiation in bone and cartilage; production of biomaterials for association with L-PRP: HA sponges (solid and injectable formulations); HA sponge with attached calcitonin; topical patches of HA sponges and L-PRP to reduce inflammation and joint pain relief. All the studied biomaterials will be characterized by their physicochemical properties (swelling, stability and transdermal permeation-patches), mechanical (rheology and injection force) and biological (release of growth factors and cytokines, and cell capabilities to proliferation and differentiation). This project will support 2 post-doctoral research works, 2 PhD thesis and 1 master dissertation, which will contribute to the formation of human resources and development of regenerative orthopedics. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOMES DE MELO, BRUNA ALICE; FRANCA, CARLA GIOMETTI; DAVILA, JOSE LUIS; BATISTA, NILZA ALZIRA; CALIARI-OLIVEIRA, CAROLINA; D'AVILA, MARCOS AKIRA; MALHEIROS LUZO, ANGELA CRISTINA; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine. Materials Science & Engineering C-Materials for Biological Applications, v. 109, . (15/23134-8, 16/10132-0)
GOMES DE MELO, BRUNA ALICE; MALHEIROS LUZO, ANGELA CRISTINA; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Centrifugation Conditions in the L-PRP Preparation Affect Soluble Factors Release and Mesenchymal Stem Cell Proliferation in Fibrin Nanofibers. Molecules, v. 24, n. 15, . (15/23134-8, 16/10132-0)
GOMES DE MELO, BRUNA ALICE; ANDRADE SANTANA, MARIA HELENA. Structural Modifications and Solution Behavior of Hyaluronic Acid Degraded with High pH and Temperature. Applied Biochemistry and Biotechnology, v. 189, n. 2, p. 424-436, . (15/23134-8, 16/10132-0)
GOMES DE MELO, BRUNA ALICE; MARTINS SHIMOJO, ANDREA ARRUDA; MARCELINO PEREZ, AMANDA GOMES; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Distribution, recovery and concentration of platelets and leukocytes in L-PRP prepared by centrifugation. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 161, p. 288-295, . (15/23134-8, 14/27200-2, 16/10132-0)
FRANCA, CARLA GIOMETTI; LEME, KRISSIA CAROLINE; MALHEIROS LUZO, ANGELA CRISTINA; HERNANDEZ-MONTELONGO, JACOBO; ANDRADE SANTANA, MARIA HELENA. Oxidized hyaluronic acid/adipic acid dihydrazide hydrogel as cell microcarriers for tissue regeneration applications. E-POLYMERS, v. 22, n. 1, p. 10-pg., . (16/10132-0)
FRANCA, CARLA GIOMETTI; SACOMANI, DANIEL PEREIRA; VILLALVA, DENISE GRADELLA; NASCIMENTO, VICENTE FRANCO; DAVILA, JOSE LUIS; ANDRADE SANTANA, MARIA HELENA. Structural changes and crosslinking modulated functional properties of oxi-HA/ADH hydrogels useful for regenerative purposes. EUROPEAN POLYMER JOURNAL, v. 121, . (16/10132-0)

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