Association studies of platelet-rich plasma with acid hyaluronic scaffolds for applications in regenerative medicine

• Grant number: 14/27200-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): January 01, 2016
• Effective date (End): February 03, 2018
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Maria Helena Andrade Santana
• Grantee: Andréa Arruda Martins Shimojo
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 17/03496-8 - Preparation of microneedles based on hyaluronic acid incorporating nanostructured lipid carriers containing resveratrol, BE.EP.PD

Abstract

The glycosaminoglycan hyaluronic acid (HA) is the main component of the synovial fluid of the joints. The functions and HA applications in health are associated primarily to its structural characteristics, and possible chemical modification of the polymer, which determine their rheological properties, solubility, hydration and specific cell recognition. In clinical practice, the association of Platelet Rich Plasma (PRP) with high molecular weight free HA has proven effective, particularly in orthopedic therapies. However, despite several reported clinical studies, basic science research aimed to characterize the molecular mechanisms involved, is still very scarce. In vitro results obtained during the PhD studies of this candidate, showed that the proliferation and osteogenic differentiation of mesenchymal stem cells (h-AdMSCs) were favored by the association of P-PRP (Platelet-Rich Plasma) with porous scaffolds (sponge) of auto-crosslinked HA (AHA) compared to free HA. Therefore, this project aims to characterize the molecular interactions between L-PRP (Leukocyte and Platelet-Rich Plasma) and scaffolds of AHA, and analyze the effects of these interactions in vitro and in vivo. Thus, different platelet activation conditions (with and without platelet agonists thrombin and Ca+2); molecular interactions between the network of fibrin and AHA using different weight ratios and reducing negative charge of the scaffold by their association with chitosan will be studied. In vivo assays will be directed to the regeneration of bone lesions, cartilage, and wound healing. The results from this work will be essential for the technological development of scaffolds for AHA-based association with PRP. In addition, the results will contribute to the standardization of PRP/ AHA association on scientific grounds.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES DE MELO, BRUNA ALICE; MARTINS SHIMOJO, ANDREA ARRUDA; MARCELINO PEREZ, AMANDA GOMES; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Distribution, recovery and concentration of platelets and leukocytes in L-PRP prepared by centrifugation. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 161, p. 288-295, JAN 1 2018. (15/23134-8, 14/27200-2, 16/10132-0)

SHIMOJO, ANDREA A. M.; FERNANDES, V, ANA RITA; FERREIRA, NUNO R. E.; SANCHEZ-LOPEZ, ELENA; SANTANA, MARIA H. A.; SOUTO, ELIANA B.. Evaluation of the Influence of Process Parameters on the Properties of Resveratrol-Loaded NLC Using 2(2) Full Factorial Design. ANTIOXIDANTS, v. 8, n. 8, AUG 2019. (14/27200-2)

MARTINS SHIMOJO, ANDREA ARRUDA; SANTOS DUARTE, ADRIANA DA SILVA; SANTOS DUARTE LANA, JOSE FABIO; MALHEIROS LUZO, ANGELA CRISTINA; FERNANDES, ANA RITA; SANCHEZ-LOPEZ, ELENA; SOUTO, ELIANA BARBOSA; ANDRADE SANTANA, MARIA HELENA. Association of Platelet-Rich Plasma and Auto-Crosslinked Hyaluronic Acid Microparticles: Approach for Orthopedic Application. POLYMERS, v. 11, n. 10, OCT 2019. (14/27200-2)