The glycosaminoglycan hyaluronic acid (HA) is the main component of the synovial fluid of the joints. The functions and HA applications in health are associated primarily to its structural characteristics, and possible chemical modification of the polymer, which determine their rheological properties, solubility, hydration and specific cell recognition. In clinical practice, the association of Platelet Rich Plasma (PRP) with high molecular weight free HA has proven effective, particularly in orthopedic therapies. However, despite several reported clinical studies, basic science research aimed to characterize the molecular mechanisms involved, is still very scarce. In vitro results obtained during the PhD studies of this candidate, showed that the proliferation and osteogenic differentiation of mesenchymal stem cells (h-AdMSCs) were favored by the association of P-PRP (Platelet-Rich Plasma) with porous scaffolds (sponge) of auto-crosslinked HA (AHA) compared to free HA. Therefore, this project aims to characterize the molecular interactions between L-PRP (Leukocyte and Platelet-Rich Plasma) and scaffolds of AHA, and analyze the effects of these interactions in vitro and in vivo. Thus, different platelet activation conditions (with and without platelet agonists thrombin and Ca+2); molecular interactions between the network of fibrin and AHA using different weight ratios and reducing negative charge of the scaffold by their association with chitosan will be studied. In vivo assays will be directed to the regeneration of bone lesions, cartilage, and wound healing. The results from this work will be essential for the technological development of scaffolds for AHA-based association with PRP. In addition, the results will contribute to the standardization of PRP/ AHA association on scientific grounds.
News published in Agência FAPESP Newsletter about the scholarship: