Influence of the association between platelet-rich plasma and hyaluronic acid on the release of growth factors, cellular proliferation and osteogenic/ chondrogenic differentiation

• Grant number: 15/23134-8
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2016
• Effective date (End): April 30, 2019
• Field of knowledge: Engineering - Chemical Engineering
• Principal researcher: Maria Helena Andrade Santana
• Grantee: Bruna Alice Gomes de Melo
• Home Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 17/02913-4 - Microfluidics for 3D h-ADMCs culture in hyaluronic acid free or structured in sponges: chemotaxis and chemokinesis in response to growth factors gradients from L-PRP, BE.EP.DR

Abstract

The platelet-rich plasma (PRP) is an autologous product obtained from the whole blood, which has been clinically used in various fields of regenerative medicine, because of its ability to stimulate tissue regeneration. In the modern classification of the blood concentrates, the P-PRP (pure-PRP, which is rich in platelets and poor in leukocytes) and L-PRP (rich in platelets and leukocytes) are the most commonly used in clinical therapies. Recent studies have shown that the balance between anabolic effects (platelets) and catabolic effects (leukocytes) in L-PRP is responsible for the regulation of cellular events on tissue regeneration. Hyaluronic acid (HA) is a glycosaminoglycan present in soft tissues and joint fluid. Its importance in biological process includes all molecular weight range, 103-106 Da. Clinical uses of L-PRP and HA of high molecular weight have shown many benefits in bone tissues regeneration. However, fundamental studies in vitro are still scarce and therefore, necessary to characterize their association and biological effects. In this context, the present work aims to prepare and characterize the association between L-PRP/HA as a new product, and investigate their effects on human adipose-derived mesenchymal stem cells (h-AdMSCs) proliferation and osteogenic/ chondrogenic differentiation, aiming standardization. Furthermore, this project proposes the use of microfluidics technology for cell migration studies, in response to L-PRPs releasate gradients. The work should be conducted in the LDPB/UNICAMP with the collaboration of the Khademhosseini Lab-Harvard/MIT, USA. L-PRPs of three ratios platelets/ leukocytes (>1, =1 e <1) and HAs of 103, 105 e 106 Da will be studied. This work has technological (preparation of autologous product L-PRP/ HA) and clinical (orthopedic applications) relevance. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOMINGOS CAVALCANTI, ANDRE DELANO; DE MELO, ALICE GOMES; MOREIRA FERREIRA, BRUNO ARMENIO; ANDRADE SANTANA, MARIA HELENA. Performance of the main downstream operations on hyaluronic acid purification. Process Biochemistry, v. 99, p. 160-170, DEC 2020. (15/23134-8)

GOMES DE MELO, BRUNA ALICE; MARTINS SHIMOJO, ANDREA ARRUDA; MARCELINO PEREZ, AMANDA GOMES; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Distribution, recovery and concentration of platelets and leukocytes in L-PRP prepared by centrifugation. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 161, p. 288-295, JAN 1 2018. (15/23134-8, 14/27200-2, 16/10132-0)

CAVALCANTI, ANDRE D. D.; MELO, BRUNA A. G.; OLIVEIRA, RHELVIS C.; SANTANA, MARIA H. A.. Recovery and Purity of High Molar Mass Bio-hyaluronic Acid Via Precipitation Strategies Modulated by pH and Sodium Chloride. Applied Biochemistry and Biotechnology, v. 188, n. 2, p. 527-539, JUN 2019. (15/23134-8)

GOMES DE MELO, BRUNA ALICE; ANDRADE SANTANA, MARIA HELENA. Structural Modifications and Solution Behavior of Hyaluronic Acid Degraded with High pH and Temperature. Applied Biochemistry and Biotechnology, v. 189, n. 2, p. 424-436, OCT 2019. (15/23134-8, 16/10132-0)

GOMES DE MELO, BRUNA ALICE; MALHEIROS LUZO, ANGELA CRISTINA; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Centrifugation Conditions in the L-PRP Preparation Affect Soluble Factors Release and Mesenchymal Stem Cell Proliferation in Fibrin Nanofibers. Molecules, v. 24, n. 15, AUG 2019. (15/23134-8, 16/10132-0)

GOMES DE MELO, BRUNA ALICE; FRANCA, CARLA GIOMETTI; DAVILA, JOSE LUIS; BATISTA, NILZA ALZIRA; CALIARI-OLIVEIRA, CAROLINA; D'AVILA, MARCOS AKIRA; MALHEIROS LUZO, ANGELA CRISTINA; SANTOS DUARTE LANA, JOSE FABIO; ANDRADE SANTANA, MARIA HELENA. Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine. Materials Science & Engineering C-Materials for Biological Applications, v. 109, APR 2020. (16/10132-0, 15/23134-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MELO, Bruna Alice Gomes de. L-PRP standardization and formation of semi-interpenetrating polymer networks of hyaluronic acid and fibrin form L-PRP for chondrogenic and osteogenic differentiation of human adipose-derived mesenchymal stem cells. 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química Campinas, SP.