Performance of the main downstream operations on hyaluronic acid purification

Author(s)

Domingos Cavalcanti, Andre Delano ^[1] ; de Melo, Alice Gomes ^[1] ; Moreira Ferreira, Bruno Armenio ^[1] ; Andrade Santana, Maria Helena ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, Dev Biotechnol, Microbial Proc Lab, BR-13083852 Campinas, SP - Brazil

• Document type: Review article
• Source: Process Biochemistry; v. 99, p. 160-170, DEC 2020.
• Web of Science Citations: 0

Abstract

Hyaluronic acid (HA), or hyaluronan, is a natural polyelectrolyte, ubiquitous in human tissues. Exogenous HA has been a valuable material due to its wide range of medical applications, such as in osteoarthritis treatment, ophthalmic surgery, adhesion prevention after surgeries and wound healing, as well as cosmetic applications. However, to ensure the physicochemical and biological properties, a purity near to 99 % is a primary requirement, aiming clinical applications. To achieve this goal, various downstream operations have been used, aiming HA concentration, separation and purification. Precipitation with organic solvents has been a common operation in most purification processes, combined with other downstream operations such as precipitation with quaternary salts, filtration, adsorption and ion exchange. This work presents an updated review of HA purification, emphasizing the performance of the main downstream operations used to achieve highly purified HA, in the period from 1970 to 2019. We conclude that, in the majority of the published works, there is a lack of studies regarding the operational conditions, as well as an absence of the purification percentage development during the processes. (AU)

• FAPESP's process: 15/23134-8 - Influence of the association between platelet-rich plasma and hyaluronic acid on the release of growth factors, cellular proliferation and osteogenic/ chondrogenic differentiation
• Grantee: Bruna Alice Gomes de Melo
• Support Opportunities: Scholarships in Brazil - Doctorate