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Potential for the use of ultrasound cavitation to extract high-purity hyaluronic acid from egg shells and process yield


Due to its excellent physical, chemical and biological characteristics, hyaluronic acid (HA) has been indicated as an ideal material for different types of treatment in the medical, pharmaceutical, dental and veterinary areas, besides being the main raw material for antiaging products and in the food industry. Commercially, HA is produced from the ridge of chicken or by bacterial fermentation. HA extracted from egg shell appears to be purer and safer for human consumption because it has chemical composition similar to human umbilical cord and medical grade purity. This extraction process is composed of the decalcification, separation, purification and filtration steps and can be generically described as decalcification assisted extraction (DAE). As this form of extraction is already patented and looking for an innovative opportunity, this project proposes the obtaining of HA from the eggshell by ultrasound assisted extraction (UAE), since this technology has been used to extract fragrances, pigments, antioxidants and compounds Organic animal tissue, yeast, food, plankton and vegetable, but was never used to extract AH from eggshell. In addition to the novelty, theoretically the UAE has the potential to produce AH without the use of chemical solvent, and in time 24 times faster than the conventional DAE technique. To verify the innovative and patentable potential, it will be verified: 1) If ultrasonic assisted extraction (UAE) can be used to obtain hyaluronic acid from eggshell, 2) Which parameters of UAE use are more productive, 3) If the simplified ultrasound assisted extraction method (SUAE) is effective and biologically safe and, 4) If leftovers from UAE and SUAE can be used to produce hydroxyapatite. Ten experimental groups will be constituted, two of them being DAE. Ten experimental groups will be constituted: 2 DAE controls (1440 min) and separation phases, complete and simplified purification and filtration, and 8 test groups in which the extraction will be done by UAE using the accumulated potencies 20, 40, 60 and 80 MJ Which correspond to the following extraction times 24, 53, 80 and 102 min. and by phases of separation, purification and simple filtration. The amount (extraction yield) and HA quality obtained by the different processes will be evaluated by weighing and the following analytical techniques: identification, quality and concentration of extracted HA (UV-Vis Chromatography as a function of Carbazole reaction); PH evaluation; Standard chemical bonding by FTIR / ATR; Molecular mass, size and structure by HPLC / GPC and viscosity by Ubbelohde viscosimeter. The obtained data will be analyzed in an inferential and comparative way, for p <0.05. It is expected to prove the efficiency of ultrasonic cavitation to extract AH from egg shells, and to define optimized production parameters aiming to obtain high value-added AH and high profitability obtained from the disposal of eggs from the food industry and farms. (AU)

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