Efficacy, mineral content and biocompatibility of an experimental low-concentrated bleaching agent composed of hyaluronic acid and titanium dioxide nanoparticles co-doped with N and F.

• Grant number: 22/04618-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2023
• End date: September 30, 2024
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Vanessa Cavalli Gobbo
• Grantee: Marcos Roberto de Lima Benati
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

This study will evaluate the efficacy, mineral content, and biocompatibility (in vitro) of an experimental bleaching agent composed of hyaluronic acid (HA), NF_TiO2 nanoparticles (NPs), and low concentrations of hydrogen peroxide (HP). This project will be divided into two studies. In study 1, sound enamel specimens will be treated with: HP (hydrogen peroxide 35%, commercial control), (PH 6% Nano White DMC, commercial control); Carbomer 940 + PH (1,5 e 6%) + NF_TiO2 (5%); AH + PH (1,5 e 6%) + NF_TiO2 (5%); HA + HP (1.5, 3, and 6%) + NF_TiO2 (0, 5, and 10%), with and without LED light violet, and a negative control (no treatment). Bleaching will be performed in 3 sessions of 30 min with 7-days intervals. The following analysis will be performed: Phase 1: Characterization of experimental gels [pH measurement, particle size (polydispersity index, PdI), particle stability (zeta potential, PZ), and viscosity]; Phase 2: Color evaluation [spectrophotometer (L*, a*, b* parameters), whitening index (WID) and color change (”E)]; Phase 3: Mineral content [surface microhardness (KHN), energy dispersive spectroscopy (EDS)]; Phase 4: Surface Morphology [two-dimensional mean roughness (Ra) and scanning electron microscopy (SEM)]. Study 2 will evaluate the biocompatibility of MDPC-23 cells submitted to hyaluronic acid-containing experimental bleaching agents, NF_TiO2 NPs, and low-concentrated HP. In Study 2 MTT test will determine the metabolism of MDPC-23 cells after treatments (Phase 1). Besides, the concentration of PH present in the cell extract will be quantified (Phase 2) and the morphology of MDPC-23 cells will be determined after bleaching treatment with experimental gels (Phase 3). The results will be statistically analyzed, and the significant level will be set at 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BENATI, MARCOS ROBERTO DE LIMA; DE MELO, PRISCILA BORGES GOBBO; RIBEIRO, RAFAEL ANTONIO DE OLIVEIRA; FLOREZ, FERNANDO LUIS ESTEBAN; COSTA, CARLOS ALBERTO DE SOUZA; CAVALLI, VANESSA. Hyaluronic Acid-Based Bleaching Gels With NF_TiO₂ and Violet LED: Efficacy and Cytotoxicity of Low-Concentration H₂O₂ for In-Office Bleaching. Journal of Esthetic and Restorative Dentistry, v. 37, n. 9, p. 10-pg., 2025-05-07. (20/06782-4, 22/04618-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BENATI, Marcos Roberto de Lima. Characterization, effects on the enamel properties and biocompatibility of a bleaching gel containing hyaluronic acid, nanoparticles and irradiated with violet LED light. 2024. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba (FOP) Piracicaba, SP.