Distribution, recovery and concentration of platelets and leukocytes in L-PRP prepared by centrifugation

Author(s)

Gomes de Melo, Bruna Alice ^[1] ; Martins Shimojo, Andrea Arruda ^[1] ; Marcelino Perez, Amanda Gomes ^[1] ; Santos Duarte Lana, Jose Fabio ^[2] ; Andrade Santana, Maria Helena ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, Campinas, SP - Brazil

^[2] IOC, Bone & Cartilage Inst, Indaiatuba, SP - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 161, p. 288-295, JAN 1 2018.
• Web of Science Citations: 4

Abstract

Platelet-rich plasma (PRP) is an autologous product prepared from whole blood (WB) that is widely used in regenerative medicine. In clinical practice, discontinuous centrifugation is used for both hand- and machine-prepared PRP. However, separation of WB fractions via centrifugation is a complex process, and the lack of clear mechanisms limits the understanding and evaluation of PRP preparation methods This paper focuses on the distribution, recovery and concentration factor of platelets and leukocytes in L-PRP (leukocyte and platelet-rich plasma) to define a concentration pattern for these blood components due to centrifugation conditions. WB collected from three healthy donors was centrifuged for 10 min at 50-800 xg in a first step and then at 400 xg in a second step. The results from the first centrifugation step showed most platelets to be distributed in the upper layer (UL) and the buffy coat (BC), with approximately 14.5 +/- 5.2% retained in the bottom layer (BL). Most leukocytes were present in the BL. The greatest platelet ``recoveries from L-PRP were obtained at up to 150 xg (88.5 +/- 16.9%). The cumulative concentration factors with respect to the WB from the second centrifugation step were 6 and 1.2 for platelets and leukocytes, respectively. Thus, the concentration patterns delineated three centrifugation ranges with platelet/leukocyte ratios of 205 +/- 18, 325 +/- 15 and 107 +/- 4 and lymphocyte/granulocyte ratios of 1.54 +/- 0.74, 0.90 +/- 0.08 and 0.42 +/- 0.07. These findings contribute to a scientifically based standardization of L-PRP preparations. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/23134-8 - Influence of the association between platelet-rich plasma and hyaluronic acid on the release of growth factors, cellular proliferation and osteogenic/ chondrogenic differentiation
• Grantee: Bruna Alice Gomes de Melo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/27200-2 - Association studies of platelet-rich plasma with acid hyaluronic scaffolds for applications in regenerative medicine
• Grantee: Andréa Arruda Martins Shimojo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/10132-0 - Platelet-rich plasma associated to hyaluronic acid for applications in regenerative orthopedics: from the technological development of preparations to physicochemical and in vitro biological characterizations
• Grantee: Maria Helena Andrade Santana
• Support Opportunities: Regular Research Grants