Preparation, modulation and standardization of platelet rich plasma for application in regenerative therapy

• Grant number: 10/11758-3
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2011
• End date: August 31, 2013
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Maria Helena Andrade Santana
• Grantee: Maria Helena Andrade Santana
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Amélia Rodrigues ; Ângela Cristina Malheiros Luzo ; Jose Fabio Santos Duarte Lana ; William Dias Belangero

Abstract

Platelet Rich Plasma (PRP) is an autologous therapy, non-immunogenic, which can stimulate and accelerate tissue regeneration. Several clinical studies have shown that PRP and growth factors are considered potent stimulant for soft tissue regeneration, since they are involved in the angiogenic process and extracellular matrix and bone tissue reconstruction. Despite the intense use of this therapy in orthopedic medicine, scientific data related to analysis and /or standardization of PRP preparation are still very dispersed.Based on this, this study aims to prepare, adjust and optimize the PRP quality as a function of the main operational variables involved in the stages of its preparation, and standardize it in their best conditions for clinical application. The study will be developed by applying the statistical technique of factorial design and response surface analysis, to the following steps, with their respective independent variables (v.i.): 1. Reception of the collected blood (vi: type and concentration of anticoagulants), 2. Preparation of PRP, which will be done in two sub-steps: 2.1. Centrifugation (vi: speed, time and temperature of centrifugation ) and 2.2. Platelet Activation (type and concentration of platelet agonists and aggregation time).The response variable, quality of PRPs, will be characterized by platelets counting, P-selectin quantification and release of growth factors .The response surfaces will allow modulate the PRP quality in differente regions, with the possibility of optimization (maximization of the response variable) as a function of the variables involved. Once delineated such modulation and optimization, will also be evaluate the influence of these PRPs in cell proliferation and differentiation-inducing ability by cultivating cells in vitro.In the regions of interest for clinical application, will be proposed a PRPs classification, with standardization of its preparation.This strategy will be applied to PRPs preparation with and without leukocyte layer. The results obtained with this study can contribute to a safe and reproductive preparation of PRPs, and it can provide a more careful classification of their quality and a better results analysis of its clinical application. (AU)