Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV

Autor(es)

Fernandes Mantoanelli, Jessica Oliveira ^[1] ; Goncalves, Luis Moreira ^[2] ; Pereira, Elisabete Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, Dept Fis Quim & Matemat, Sorocaba, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chromatographia; v. 83, n. 6 APR 2020.
• Citações Web of Science: 0

Resumo

Biogenic amines (BAs) are important compounds that can be used in the quality control of food and beverages. BA analysis is a challenging task that can be made easier by applying a derivatizing agent like dansyl chloride (DNS). The optimized capillary zone electrophoresis (CZE) separation of the DNS-BA derivates (derivates of cadaverine, histamine, putrescine, tryptamine, and tyramine) was performed using benzylamine as an internal standard, a potential of 18 kV, a temperature of 23 degrees C, a running buffer consisting of phosphoric acid, 120 mmol L-1, pH 2.5, and an hydrodynamic injection at 25 mBar for 6 s. All calibration curves had r(2) higher than 0.99, and limits of detection (LODs) ranged from 7 to 50 mu g L-1. The developed methodology was tested in cheese and yogurt samples. DNS showed to be an alternative derivatization reagent not only because it produced UV-detectable derivates (214 nm), but also because of its stability, aqueous solubility, high selectivity and sensitivity, reduced impurities, and simple preparation steps. (AU)

• Processo FAPESP: 18/14425-7 - Um novo rumo sinérgico para a microextração por difusão gasosa (GDME) fazendo uso de polímeros com impressão molecular (MIPs)
• Beneficiário: Luís Francisco Moreira Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/03582-7 - Eletroforese capilar com técnica analítica para a determinação de aminas biogênicas em matrizes de alimentos
• Beneficiário: Elisabete Alves Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular