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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV

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Author(s):
Fernandes Mantoanelli, Jessica Oliveira [1] ; Goncalves, Luis Moreira [2] ; Pereira, Elisabete Alves [1]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Carlos UFSCar, Dept Fis Quim & Matemat, Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Chromatographia; v. 83, n. 6 APR 2020.
Web of Science Citations: 0
Abstract

Biogenic amines (BAs) are important compounds that can be used in the quality control of food and beverages. BA analysis is a challenging task that can be made easier by applying a derivatizing agent like dansyl chloride (DNS). The optimized capillary zone electrophoresis (CZE) separation of the DNS-BA derivates (derivates of cadaverine, histamine, putrescine, tryptamine, and tyramine) was performed using benzylamine as an internal standard, a potential of 18 kV, a temperature of 23 degrees C, a running buffer consisting of phosphoric acid, 120 mmol L-1, pH 2.5, and an hydrodynamic injection at 25 mBar for 6 s. All calibration curves had r(2) higher than 0.99, and limits of detection (LODs) ranged from 7 to 50 mu g L-1. The developed methodology was tested in cheese and yogurt samples. DNS showed to be an alternative derivatization reagent not only because it produced UV-detectable derivates (214 nm), but also because of its stability, aqueous solubility, high selectivity and sensitivity, reduced impurities, and simple preparation steps. (AU)

FAPESP's process: 18/14425-7 - A new synergetic path for gas-diffusion microextraction (GDME) making use of molecular imprinted polymers (MIPs)
Grantee:Luís Francisco Moreira Gonçalves
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 19/03582-7 - Capillary electrophoresis as analytical technique for the determination of biogenic amines in food matrices
Grantee:Elisabete Alves Pereira
Support Opportunities: Regular Research Grants