Effects of extracellular vesicles released by peritoneal B-1 cells on experimental Leishmania (Leishmania) amazonensis infection

Autor(es)

Toledo, Mayte dos Santos ^[1] ; Cronemberger-Andrade, Andre ^[2] ; Barbosa, Fernanda Marins Costa ^[1] ; Reis, Natasha Ferraz de Campos ^[1] ; Dupin, Talita Vieira ^[1] ; Soares, Rodrigo Pedro ^[3] ; Torrecilhas, Ana Claudia ^{[1, 2]} ; Xander, Patricia ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo Campus Diadema, Dept Pharmaceut Sci, Diadema, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Med Infect Dis, Sao Paulo - Brazil

^[3] Fundacao Oswaldo Cruz, Rene Rachou Inst, Lab Cellular & Mol Parasitol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Leukocyte Biology; v. 108, n. 6 APR 2020.
• Citações Web of Science: 7

Resumo

B-1 cells are a B-lymphocyte subtype whose roles in immunity are not completely defined. These cells can produce cytokines (mainly IL-10) and natural and specific antibodies. Currently, extracellular vesicles (EVs) released by immune cells have emerged as new important entities in cell-cell communication. Immune cells release EVs that can activate and/or modulate other immune cells. Here, we characterized the EVs released by peritoneal B-1 cells infected or not with Leishmania (Leishmania) amazonensis. This Leishmania species causes cutaneous leishmaniasis and can infect macrophages and B-1 cells. Our results showed that peritoneal B-1 cells spontaneously release EVs, but the parasite stimulated an increase in EVs production by peritoneal B-1 cells. The treatment of BALB/c and C57BL/6 bone marrow-derived macrophages (BMDM) with EVs from infected peritoneal B-1 cells led to differential expression of iNOS, IL-6, IL-10, and TNF-alpha. Additionally, BALB/c mice previous treated with EVs released by peritoneal B-1 cells showed a significant lower lesion size and parasite burden. Thus, this study demonstrated that peritoneal B-1 cells could release EVs that can alter the functions of macrophages in vitro and in vivo these EVs altered the course of L. amazonensis infection. These findings represent the first evidence that EVs from peritoneal B-1 cells can act as a new mechanism of cellular communication between macrophages and B-1 cells, contributing to immunity against experimental leishmaniasis. (AU)

• Processo FAPESP: 14/26510-8 - Estudo do efeito de fatores liberados por linfócitos B-1 infectados com L (L.) amazonensis sobre macrófagos peritoneais murinos
• Beneficiário: Maytê dos Santos Toledo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica