| Texto completo | |
| Autor(es): |
Galvao Tessaro, Fernando Henrique
[1]
;
Ayala, Thais Soprani
[1]
;
Bella, Leonardo Mendes
[1]
;
Martins, Joilson Oliveira
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo FCF USP, Dept Clin & Toxicol Anal, Lab Immunoendocrinol, Sch Pharmaceut Sci, Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Immunobiology; v. 225, n. 2 MAR 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Diabetes causes dysregulation in signal transduction in immune cells leading to an impaired response to pathogens. Herein, we investigated the impact of type 1 diabetes (T1D) in bone marrow-derived macrophages (BMDM), using male non-diabetic and diabetic C57BL/6 mice (alloxan 60 mg/kg, i.v., CEUA/FCF/USP - 467). Diabetic BMDM expressed impaired phosphoinositide 3-kinase (PI3K), being lower p-PI3K p55 levels and higher levels of PI3K p110 alpha, whereas protein kinase B (PKB/Akt) (Ser-473 and Thr-308), extracellular signalregulated kinases (ERK 1/2), and stress-activated protein kinase (SAPK/JNK) were enhanced compared to nondiabetic BMDM. Further evaluation of the responsiveness to lipopolysaccharide (LPS; 0.1 and 1 ug/mL), diabetic BMDM and peritoneal macrophage secreted dysregulated levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-10 levels. In 24 h, diabetic BMDM stimulated by LPS presented lower metabolic activity, with no differences in cell surveillance. Therefore, LPS re-stimulation (0.1 ug/mL) in diabetic BMDM resulted in higher secretion of TNF-alpha compared to non-diabetic BMDM. However, diabetic peritoneal macrophages secreted similar IL-6 levels in the first and additional 24 h of LPS stimulation. In general, our results demonstrated that diabetes exerts an impact in both BMDM and peritoneal macrophages ability to secrete cytokine under LPS stimulation. (AU) | |
| Processo FAPESP: | 14/05214-1 - Investigando o papel da insulina em diferentes infecções em animais diabéticos e sadios |
| Beneficiário: | Joilson de Oliveira Martins |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/11540-7 - Investigando o papel da insulina na vigência da inflamação alérgica pulmonar em camundongos diabéticos e sadios |
| Beneficiário: | Joilson de Oliveira Martins |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/02272-0 - Efeito da insulina na inflamação pulmonar secundária a sepse, na imunidade inata, na ativação do seu gene (bgk) e seus receptores (ir-a e ir-b) |
| Beneficiário: | Joilson de Oliveira Martins |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 19/25999-7 - Macrofagos de camundogos diabéticos tipo 1 apresentam alterações nas vias pi3k/akt, erk 1/2 e sapk/jnk |
| Beneficiário: | Joilson de Oliveira Martins |
| Modalidade de apoio: | Auxílio à Pesquisa - Publicações científicas - Artigo |