Medical applications of clustered regularly interspaced short palindromic repeats (CRISPR/Cas) tool: A comprehensive overview

Autor(es)

Araldi, Rodrigo Pinheiro ^{[1, 2]} ; Khalil, Charbel ^[3] ; Grignet, Pedro Henrique ^[4] ; Teixeira, Michelli Ramires ^[4] ; de Melo, Thatiana Correa ^[4] ; Modolo, Diego Grando ^[5] ; Virgilio Fernandes, Luis Guilherme ^[6] ; Ruiz, Jorge ^{[4, 2]} ; de Souza, Edislane Barreiros ^[7]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Morphol & Genet, Genet Bases Thyroid Tumors Lab, Escola Paulista Med, Sao Paulo, SP - Brazil

^[2] Univ Fed Integracao Latino Amer UNILA, Programa Posgrad Biociencias, Foz Do Iguacu, PR - Brazil

^[3] Lebanese Univ, Middle East Inst Hlth Univ Hosp, Rev Res & Applicat Ctr, Beirut - Lebanon

^[4] Univ Fed Integracao Latino Amer UNILA, Inst Latino Amer Ciencias Vida & Nat ILACVN, Foz Do Iguacu, PR - Brazil

^[5] Inst Butantan, Genet Lab, Sao Paulo, SP - Brazil

^[6] Inst Butantan, Special Lab Vaccine Dev, Sao Paulo - Brazil

^[7] Univ Estadual Paulista, Fac Ciencias & Letras Assis, Lab Genet Mol Biol & Mutagenesis, UNESP, Assis, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Gene; v. 745, JUN 30 2020.
• Citações Web of Science: 0

Resumo

Since the discovery of the double helix and the introduction of genetic engineering, the possibility to develop new strategies to manipulate the genome has fascinated scientists around the world. Currently scientists have the knowledge and ability to edit the genomes. Several methodologies of gene editing have been established, all of them working like ``scissor{''}, creating double strand breaks at specific spots. The introduction of a new technology, which was adapted from the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas bacterial immune system, has revolutionized the genetic therapy field, as it allows a much more precise editing of gene than the previously described tools and, therefore, to prevent and treat disease in humans. This review aims to revisit the genome editing history that led to the rediscovery of the CRISPR/Cas technology and to explore the technical aspects, applications and perspectives of this fascinating, powerful, precise, simpler and cheaper technology in different fields. (AU)

• Processo FAPESP: 14/06570-6 - Sequenciamento completo do exoma, Paired-end RNA e genoma: novos insights sobre a natureza genética do câncer de tiróide na idade adulta e na faixa etária pediátrica e aplicações na prática clínica
• Beneficiário: Janete Maria Cerutti
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/14948-7 - Estudo do papel de LIMD2 na transição epitélio-mesênquima no câncer de tiroide
• Beneficiário: Rodrigo Pinheiro Araldi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado