| Texto completo | |
| Autor(es): |
Gomes, O. P.
[1]
;
Feltran, G. S.
[2]
;
Ferreira, M. R.
[2]
;
Albano, C. S.
[2, 1]
;
Zambuzzi, W. F.
[2]
;
Lisboa-Filho, P. N.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ, Sch Sci, Dept Phys, UNESP, Bauru, SP - Brazil
[2] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Chem & Biochem, UNESP, Botucatu, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 190, JUN 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Surface modification of medical and dental devices, to improve their biocorrosion resistance and biocompatibility, can be achieved with the multidisciplinary field of biomaterials. Nanostructured titanium dioxide (TiO2) has been employed as surface modifier of titanium-based biomaterials because it can prevent the failure of the devices due to wear mechanisms. Moreover, this oxide surface is mostly terminated by hydroxyl groups (-OH) that can be directly functionalized with biomolecules to improve the biocompatibility of these devices. We explored the influence of 3-aminopropyltrimethoxysilane (APTMS) molecules as spacers in bovine serum albumin (BSA) protein immobilization on the physically hydroxylated surfaces of ruffle phase TiO2 films grown by reactive Radio Frequency (RF) magnetron sputtering. X-ray Photoelectron Spectroscopy (XPS) was used to examine the adsorption of BSA and APTMS on the hydroxylated surface of TiO2 thin films. For biological tests, BSA was directly immobilized on the film surface and on the APTMS monolayer. Biological analysis found better osteoblast performance considering gene markers related to cell adhesion after interacting directly with the surface modified by the immobilization of BSA, especially on the surface where this protein was immobilized by APTMS. Additionally, we addressed the relevance of this biointerfaces on extracellular matrix remodeling by zymography analysis. Altogether, our data provides new insights about the cellular and molecular mechanisms covering the improved osteoblastic response of the proposed surface modification. (AU) | |
| Processo FAPESP: | 16/22186-7 - Interação de Proteínas em Superfícies de Filmes Finos de TiO2 |
| Beneficiário: | Orisson Ponce Gomes |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo |
| Beneficiário: | Willian Fernando Zambuzzi |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 17/15035-5 - Adsorção de bisfosfonatos e proteínas em superfícies de TiO2 para implantes ósseos |
| Beneficiário: | Paulo Noronha Lisboa Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 14/20471-0 - Filmes finos de óxidos metálicos funcionalizados para estudo da adsorção de proteínas |
| Beneficiário: | Paulo Noronha Lisboa Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |