Heat stress effects on bovine sperm cells: a chronological approach to early findings

Autor(es):Mostrar menos -

Garcia-Oliveros, Laura Nataly ^[1] ; de Arruda, Rubens Paes ^[2] ; Batissaco, Leonardo ^[1] ; Guilger Gonzaga, Vitor Hugo ^[1] ; Moreira Nogueira, Vinicius Jose ^[1] ; Florez-Rodriguez, Shirley Andrea ^[1] ; Almeida, Flavia dos Santos ^[1] ; Rodrigues Alves, Maira Bianchi ^[1] ; Costa Pinto, Samara Cristine ^[1] ; Nichi, Marcilio ^[3] ; de Agostini Losano, Joao Diego ^[3] ; Vechiato Kawai, Giulia Kiyomi ^[3] ; Carvalho Celeghini, Eneiva Carla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Biotechnol Anim Reprod, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Teaching & Res Pathol Reprod, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Ctr Biotechnol Anim Reprod, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Pirassununga, SP - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF BIOMETEOROLOGY; v. 64, n. 8 MAY 2020.
• Citações Web of Science: 2

Resumo

Testicular heat stress affects sperm quality and fertility. However, the chronology of these effects is not yet fully understood. This study aimed to establish the early sequential effects of heat stress in bull sperm quality. Semen and blood samples of Nellore breed bulls were collected and distributed into control and testicular heat stress (scrotal bags/96 h) groups. Semen samples were evaluated for sperm motility, abnormalities, plasma membrane integrity, acrosomal membrane integrity, mitochondrial membrane potential, sperm lipid peroxidation, seminal plasma lipid peroxidation, and DNA fragmentation. Blood plasma was also evaluated for lipid peroxidation. An increase in sperm abnormalities was observed 7 days following heat stress. After 14 days, sperm lipid peroxidation increased and mitochondrial membrane function, sperm motility, and plasma membrane integrity decreased. Heat stress effects were still observed after 21 days following heat stress. An increase in sperm DNA fragmentation was observed as a late effect after 28 days. Thus, the initial effects of heat stress (i.e., increasing sperm abnormalities and lipid peroxidation) suggest the presence of oxidative stress in the semen that alters mitochondrial function, sperm motility, plasma membrane integrity, and belatedly, DNA fragmentation. Although sperm abnormalities persisted and increased over time, sperm lipid peroxidation, in turn, increased only until 21 days after heat stress. In this regard, these findings provide a greater understanding of the chronological effects of experimentally induced heat stress on bovine sperm, providing valuable insights about spermatogenesis during the first 28 days following heat stress. (AU)

• Processo FAPESP: 14/12757-1 - Perfil metabólico, hormonal e de proteínas de fase aguda em vacas prenhes e vazias e sua relação com hemodinâmica uterina e qualidade do sêmen
• Beneficiário: Eneiva Carla Carvalho Celeghini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/05395-1 - Perfil de microRNAs presentes no sêmen e embriões bovinos e sua relação com a fertilidade
• Beneficiário: Eneiva Carla Carvalho Celeghini
• Modalidade de apoio: Auxílio à Pesquisa - Regular