Detection of Cyanotoxin-Producing Genes in a Eutrophic Reservoir (Billings Reservoir, SAo Paulo, Brazil)

Autor(es)

Ribeiro, Matheus S. F. ^{[1, 2]} ; Tucci, Andrea ^[3] ; Matarazzo, Matheus P. ^[1] ; Viana-Niero, Cristina ^[2] ; Nordi, Cristina S. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Ciencias Ambientais, Rua Prof Artur Riedel 275, Diadema 09972270 - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Rua Botucatu 862, Sao Paulo 04023901 - Brazil

^[3] Inst Bot, Ctr Pesquisa Plantas Avasculares & Fungos, Nucleo Pesquisa Ficol, Av Miguel Estefano 3687, Sao Paulo 04301012 - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WATER; v. 12, n. 3 MAR 2020.
• Citações Web of Science: 0

Resumo

CyanoHABs (cyanobacterial harmful algal blooms) are blooms of cyanobacteria capable of producing cyanotoxins, a large group of secondary metabolites that are toxic to most eukaryotes. In this work, the main aim was to evaluate the presence of multiple genes from each of the clusters responsible for biosynthesis of cyanotoxins (cylindrospermopsin, microcystin and saxitoxin) in total DNA obtained from sixteen environmental water samples by PCR. Microcystin gene mcyE was amplified in all analyzed samples. Among the cylindrospermopsin genes analyzed, only the cyrC gene was amplified from DNA obtained from three of sixteen samples. Of the three different saxitoxin genes analyzed, sxtB and sxtI were present in four and three of the sixteen samples studied, respectively, and sxtA did not show any positive result. Based on our results, we suggest caution when using only one gene from the full clusters responsible for biosynthesis of cyanotoxins, given that it may not be sufficient to confirm or exclude the toxigenic potential of a sample. (AU)

• Processo FAPESP: 10/50738-8 - Monitoramento intensivo de reservatórios da região metropolitana de São Paulo, com ênfase nas cianobactérias e sua correlação com parâmetros físicos e químicos: o caso da Billings
• Beneficiário: Cristina Souza Freire Nordi
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Processo FAPESP: 10/52641-1 - Estudo da biodiversidade de micobactérias ambientais isoladas de água bruta e de sistema de distribuição da Fundação Parque Zoológico de São Paulo
• Beneficiário: Cristina Viana Niero
• Modalidade de apoio: Auxílio à Pesquisa - Regular