The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples

Autor(es):Mostrar menos -

Pinto, Ana P. ^[1] ; Vieira, Tales S. ^[2] ; Marafon, Bruno B. ^[3] ; Batitucci, Gabriela ^[2] ; Cabrera, Elisa M. B. ^[4] ; da Rocha, Alisson L. ^[1] ; Kohama, Eike B. ^[1] ; Rodrigues, Kellen C. C. ^[5] ; de Moura, Leandro P. ^[5] ; Pauli, Jose R. ^[5] ; Cintra, Dennys E. ^[5] ; Ropelle, Eduardo R. ^[5] ; de Freitas, Ellen C. ^{[2, 3]} ; da Silva, Adelino S. R. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto 14049900, SP - Brazil

^[2] State Univ Sao Paulo Julio de Mesquita Filho Arar, Postgrad Program Nutr Sci, Araraquara 14800903, SP - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto 14040907, SP - Brazil

^[4] Univ Guadalajara, Hlth Sci Univ Ctr, Inst Translat Nutrigenet & Nutrigen, Dept Mol Biol & Genom, Guadalajara 44100, Jalisco - Mexico

^[5] Univ Estadual Campinas, UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira 13484350, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 12, n. 3 MAR 2020.
• Citações Web of Science: 0

Resumo

The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes. (AU)

• Processo FAPESP: 17/09038-1 - Efeitos do exercício físico regular e do overtraining no comportamento da via autofágica em diferentes tecidos de camundongos.
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular