Metabolic responses in bullfrog, Lithobates catesbeianus after exposure to zinc, copper and cadmium

Autor(es)

Chagas, B. R. C. ^[1] ; Utsunomiya, H. S. M. ^[2] ; Fernandes, M. N. ^[3] ; Carvalho, C. S. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Programa Posgrad Biotecnol & Monitoramento Ambien, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Caixa Postal 676, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 233, JUL 2020.
• Citações Web of Science: 0

Resumo

This study investigated the activity of lactated dehydrogenase (LDH), malate dehydrogenase (MDH) enzymes and the levels of glucose, protein and triglyceride in bullfrog tadpoles after exposure to 1 mu g L-1 of zinc (Zn), copper (Cu) and cadmium (Cd) isolated and combined for 2 and 16 days. Zn, Cu + Cd and Zn + Cu + Cd increased the activity of the LDH (2 and 16 days) and MDH (2 days) enzymes in the liver; and MDH increased in the kidney after 16 days in all co-exposed groups compared to the control. Glucose increased in the liver in the Zn and Cu groups at 2 and 16 days of exposure and decreased in the kidney (groups Cd, Zn + Cd and Cu + Cd) and muscle (Cd) at 2 days of exposure. After 2 days of exposure, the protein increased in the liver (Zn), in the kidney in all groups exposed to metals except in the groups exposed to Cd and Zn + Cu + Cd, which did not change and decreased in muscle in all the groups exposed to isolated metals. Regarding triglycerides, the kidney and muscle were the most affected, leading to a decrease in the Zn, Cu and Cd groups and in the Zn + Cu (16 days) and Zn + Cu + Cd groups (2 days). The anaerobiosis and aerobiosis were activated in the liver and kidney after short-term exposure (2 days) and in the kidney, the aerobic metabolism was activated after chronic exposure (16 days). The metals caused toxicity and were higher in co-exposure to metals with a potential to cause metabolism damage in L. catesbeianus. (AU)

• Processo FAPESP: 17/23781-9 - Avaliação ecotoxicológica da água do rio Sorocaba utilizando uma análise integrada de biomarcadores bioquímicos e morfológicos em girinos de rã-touro, Lithobates catesbeianus (Shaw, 1802).
• Beneficiário: cleoni dos santos carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50752-3 - Biomarcadores de exposicao a metais em girinos de ra-touro.
• Beneficiário: cleoni dos santos carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular