Transferable Resistance Gene optrA in Enterococcus faecalis from Swine in Brazil

Autor(es):Mostrar menos -

Almeida, Lara M. ^{[1, 2]} ; Lebreton, Francois ^{[3, 4]} ; Gaca, Anthony ^{[3, 4]} ; Bispo, Paulo M. ^{[3, 4]} ; Saavedra, Jose T. ^{[3, 4]} ; Calumby, Rodrigo N. ^[2] ; Grillo, Luciano M. ^[2] ; Nascimento, Ticiano G. ^[2] ; Filsner, Pedro H. ^[5] ; Moreno, Andrea M. ^[5] ; Gilmore, Michael S. ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP - Brazil

^[2] Univ Fed Alagoas, Inst Pharmaceut Sci, Maceio, Alagoas - Brazil

^[3] Harvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA 02115 - USA

^[4] Harvard Med Sch, Dept Ophthalmol, Boston, MA 02115 - USA

^[5] Univ Sao Paulo, Sch Vet Med & Anim Sci, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Antimicrobial Agents and Chemotherapy; v. 64, n. 6 JUN 2020.
• Citações Web of Science: 0

Resumo

OptrA is an ATP-binding cassette (ABC)-F protein that confers resistance to oxazolidinones and phenicols and can be either plasmid-encoded or chromosomally encoded. Here, we isolated 13 Enterococcus faecalis strains possessing a linezolid MIC of >= 4 mg/liter from nursery pigs in swine herds located across Brazil. Genome sequence comparison showed that these strains possess optrA in different genetic contexts occurring in 5 different E. faecalis sequence type backgrounds. The optrA gene invariably occurred in association with an araC regulator and a gene encoding a hypothetical protein. In some contexts, this genetic island was able to excise and form a covalently closed circle within the cell; this circle appeared to occur in high abundance and to be transmissible by coresident plasmids. (AU)

• Processo FAPESP: 14/27267-0 - Análise dos efeitos de inibidores da recombinação homóloga em mutações ribossômicas envolvidas com a resistência à linezolida em cocos Gram-positivos
• Beneficiário: Lara Mendes de Almeida
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado