Saline stress affects the pH-dependent regulation of the transcription factor PacC in the dermatophyteTrichophyton interdigitale

Autor(es)

da Silva, Larissa Gomes ^[1] ; Martins, Maira Pompeu ^[1] ; Sanches, Pablo Rodrigo ^[1] ; de Aguiar Peres, Nalu Teixeira ^[2] ; Martinez-Rossi, Nilce Maria ^[1] ; Rossi, Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 51, n. 4 JUN 2020.
• Citações Web of Science: 0

Resumo

Fungal growth and development depend on adaptation to the particular pH of their environment. Ambient pH sensing implies the activation of thepacCsignaling pathway, which then acts as a critical regulator for different physiological conditions. The PacC transcription factor may also be associated with the control of salt stress tolerance. In a pH-dependent manner, salinity stress is surpassed by changes in gene expression and coordinated activation of other signaling pathways, thus permitting survival in the challenging environment. In this study, we assessed the regulatory role ofTrichophyton interdigitalePacC in response to pH variation and salinity stress. By employing gene expression analysis, we evaluated the influence of PacC in the modulation of salt stress-related genes, including the transcription factorscrz1,egr2, and the MAP kinasehog1in the dermatophyteT. interdigitale. In our analysis, we also included the evaluation of a potassium/sodium efflux P-type ATPase aiming to identify the role of PacC on its ion pumping activity.Here we demonstrated that salinity stress and buffered pH conditions might affect thepacCgene modulation in the dermatophyteT. interdigitale. (AU)

• Processo FAPESP: 09/08411-4 - Caracterização funcional de genes do fungo patogênico Trichophyton rubrum durante o processo de interação com células e moléculas do microambiente hospedeiro.
• Beneficiário: Nalu Teixeira de Aguiar Peres
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/15017-8 - Caracterização funcional do fator de transcrição ACE2 do dermatófito Trichophyton rubrum e a sua implicação na interação patógeno/hospedeiro
• Beneficiário: Larissa Gomes da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/03847-7 - Caracterização molecular de mecanismos envolvidos na patogenicidade e sinalização celular em fungos
• Beneficiário: Nilce Maria Martinez-Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/11319-1 - Fatores de transcrição e sinalização celular.
• Beneficiário: Maíra Pompeu Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado