Analysis of the effect of photodynamic therapy with Fotoenticine on gliosarcoma cells

Autor(es)

Ferreira-Strixino, Juliana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Res & Dev, Lab Photobiol Appl Hlth, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 30, JUN 2020.
• Citações Web of Science: 0

Resumo

Gliosarcoma is a highly aggressive malignant neoplasm and a histopathological variant of wild-type glioblastoma multiforme isocitrate dehydrogenase (HDI). The current standard treatment consists of chemotherapy, radiotherapy and surgical resection, however, despite advances in these techniques, the patient's prognosis remains unfavorable. Photodynamic therapy (PDT) is a noninvasive technique that has been highlighted as an alternative form of cancer treatment because it does not present the side effects associated with systemic treatments. The objective of this study was to evaluate the cell viability and the intracellular localization of photosensitizer (PS) chlorin e6 Fotoenticine in 9L/lacZ cells. Therefore, tests of cytotoxicity, morphology, and location of PS were performed. The viability WA showed no cytotoxicity in the dark at all concentrations and 100 % cell death at the highest concentrations after PDT. The mitochondrial activity test showed a reduction in all groups after PDT. The production of reactive oxygen species (ROS) was higher in the PDT groups and dependent on the PS concentration. Morphological analysis after PDT showed apparent cytoplasmic destruction in all the tested concentrations, with the presence of rounded cells due to the loss of their extensions and absence of nuclear alterations. The PS accumulation in the mitochondria and cytoskeleton was observed by the confocal microscopy; however, there is no evidence of its internalization in the lysosomes. It was concluded that PDT with Fotoenticine is a promising alternative therapy showing decreased cell viability, increased ROS production and adequate localization to trigger cell death. (AU)

• Processo FAPESP: 16/20022-7 - Comparação dos efeitos da clorina e ALA aplicado à Terapia Fotodinâmica em Gliossarcoma.
• Beneficiário: Leticia Corrêa Fontana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/01469-3 - Análise do efeito da clorina E6 no tratamento de gliossarcoma, estudo in vitro
• Beneficiário: Rainara Moreno Sanches de Almeida
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/12211-4 - Estudo do efeito genotóxico, do potencial de membrana e das vias de morte celular da curcumina na terapia fotodinâmica em promastigota e amastigota de L. major e L. braziliensis: estudo in vitro
• Beneficiário: Juliana Ferreira Strixino
• Modalidade de apoio: Auxílio à Pesquisa - Regular