Miniaturized extraction method for analysis of synthetic opioids in urine by microextraction with packed sorbent and liquid chromatography-tandem mass spectrometry

Autor(es)

da Cunha, Kelly Francisco ^{[1, 2]} ; Rodrigues, Leonardo Costalonga ^{[3, 2, 4]} ; Huestis, Marilyn A. ^[5] ; Costa, Jose Luiz ^{[3, 2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, BR-13083859 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

^[4] Huestis, Marilyn A., Thomas Jefferson Univ, Inst Emerging Hlth Profess, Philadelphia, PA 19107 USA.da Cunha, Kelly Francisco, Univ Estadual Campinas, Fac Med Sci, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil

^[5] Thomas Jefferson Univ, Inst Emerging Hlth Profess, Philadelphia, PA 19107 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatography A; v. 1624, AUG 2 2020.
• Citações Web of Science: 1

Resumo

Synthetic opioids are responsible for numerous overdoses and fatalities worldwide. Currently, fentanyl and its analogs are also mixed with heroin, cocaine and methamphetamine, or sold as oxycodone, hydrocodone and alprazolam in counterfeit medications. Microextraction techniques became more frequent in analytical toxicology over the last decade. A method to simultaneously quantify nine synthetic opioids, fentanyl, sufentanil, alfentanil, acrylfentanyl, thiofentanyl, valerylfentanyl, furanylfentanyl, acetyl fentanyl and carfentanil, and two metabolites, norfentanyl and acetyl norfentanyl, in urine samples by microextraction with packed sorbent (MEPS) and liquid chromatography-tandem mass spectrometry (LCMS/MS) was developed and validated. A multivariate optimization was performed to establish the number and speed (stroke) of draw-eject sample cycles and the extraction solvent. The best extraction condition was eight draw-eject sample cycles, with a velocity of 3.6 mu L/sec and acetonitrile as elution solvent. Linearity was achieved between 1 to 100 ng/mL, with a limit of detection (LOD) of 0.1 ng/mL and limit of quantification (LOQ) of 1 ng/mL. Imprecision (% relative standard deviation) and bias (%) were less than 12.8% and 5.7%, respectively. The method had good specificity and selectivity when challenged with 10 different matrix sources and 36 pharmaceuticals and drugs of abuse at concentrations of 100 or 500 ng/mL. The method was successfully applied to authentic urine samples. MEPS was an efficient semi-automatic extraction technique, requiring small volumes of organic solvents (640 mu L) and sample (200 mu L). The cartridges can be cleaned and reused (average of 150 sample extractions/barrel inside and needle). (C)2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/11849-0 - A toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de fluido oral e caracterização de seu metabolismo in vitro
• Beneficiário: Kelly Francisco da Cunha
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/24515-0 - Desenvolvimento de método de microextração com sorbente empacotado (MEPS) para análise de opióides sintéticos em amostras de urina
• Beneficiário: Leonardo Costalonga Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/00432-1 - A Toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de cabelo e fluido oral
• Beneficiário: José Luiz da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular